PerCP荧光/Cy5.5® Anti-PD-L1抗体[29E.2A3] (ab272349)
![Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)](https://www.abcam.cn/ps/products/272/ab272349/Images/ab272349-387926-AntiPDL1-antibody-29E2A3-flowcytometry-monocytes-human.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)")
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [29E.2A3] to PD-L1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
PerCP荧光/Cy5.5® Anti-PD-L1抗体[29E.2A3]
参阅全部 PD-L1 一抗 -
描述
PerCP荧光/Cy5.5®小鼠单克隆抗体[29E.2A3] to PD-L1 -
宿主
Mouse -
偶联物
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human PD-L1.
Database link: Q9NZQ7 -
阳性对照
- Flow cyt: peripheral blood mononuclear cells.
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常规说明
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Size exclusion -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
29E.2A3 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272349于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
靶标
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功能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
组织特异性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane and Endomembrane system. - Information by UniProt
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数据库链接
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
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别名
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
图片
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Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)
Separation of human monocytes (Red) from cellular debris (black-dashed) in flow cytometric analysis labeling CD274 using ab272349. (4 μl reagent per milion cells in 100 μl of cell suspension).
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![Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)](https://www.abcam.cn/ps/products/272/ab272349/Images/ab272349-387923-AntiPDL1-antibody-29E2A3-flowcytometry-peripheral-blood-mononuclear-cells-human.jpg)
Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)Flow cytometric analysis of peripheral blood mononuclear cells labeling CD274 with ab272349 at 4μl reagent per milion cells in 100 μl of cell suspension. (4 μl reagent per milion cells in 100 μl of cell suspension).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272349 尚未被引用在任何文献中。