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重组RabMAb

重组Anti-PER2 (phospho S662)抗体[EPR19820] - BSA and Azide free (ab271972)

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Dot Blot - Anti-PER2 antibody [EPR19820] (ab271972)
  • Anti-PER2 (phospho S662) antibody [EPR19820] - BSA and Azide free (ab271972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19820] to PER2 (phospho S662) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-PER2 (phospho S662)抗体[EPR19820] - BSA and Azide free
    参阅全部 PER2 一抗

  • 描述

    兔单克隆抗体[EPR19820] to PER2 (phospho S662) - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Dot blotmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HEK-293T whole cell lysate transfected with hPER2.

  • 常规说明

    ab271972 is the carrier-free version of ab206377.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271972于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
Dot blot
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
Dot blot
Use at an assay dependent concentration.

靶标

  • 功能

    Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
    NPAS2-BMAL1
    BMAL2-induced transactivation.

  • 组织特异性

    Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung.

  • 疾病相关

    Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.

  • 序列相似性

    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • 翻译后修饰

    Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.

  • 细胞定位

    Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Circadian clock protein PERIOD 2 antibody
      • FASPS antibody
      • FASPS1 antibody
      • hPER 2 antibody
      • hPER2 antibody
      • KIAA0347 antibody
      • OTTHUMP00000164476 antibody
      • PER 2 antibody
      • PER2 antibody
      • PER2_HUMAN antibody
      • Period 2 antibody
      • Period 2 isoform 1 antibody
      • Period circadian clock 2 antibody
      • Period circadian protein 2 antibody
      • Period circadian protein homolog 2 antibody
      • Period homolog 2 (Drosophila) antibody
      • Period homolog 2 antibody
      • Period, Drosophila, homolog of, 2 antibody
      • Period2 antibody
      see all

    图片

    • Dot Blot - Anti-PER2 antibody [EPR19820] (ab271972)
      Dot Blot - Anti-PER2 antibody [EPR19820] (ab271972)

      Lane 1: PER2 (phospho S662) peptide labelled with ab206377 primary antibody at 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

      Lane 2: PER2 non-phospho peptide labelled with ab206377 primary antibody at 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206377).
    • Anti-PER2 (phospho S662) antibody [EPR19820] - BSA and Azide free (ab271972)
      Anti-PER2 (phospho S662) antibody [EPR19820] - BSA and Azide free (ab271972)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271972？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271972 尚未被引用在任何文献中。

    客户评价及客户问答

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