Anti-PER2抗体(ab272930)
Key features and details
- Rabbit polyclonal to PER2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PER2抗体
参阅全部 PER2 一抗 -
描述
兔多克隆抗体to PER2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Mouse PER2. The exact sequence is proprietary. Center region.
Database link: O54943 -
阳性对照
- WB: PER2 transfected HEK-293T whole cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272930于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. |
靶标
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功能
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
NPAS2-BMAL1
BMAL2-induced transactivation. -
组织特异性
Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung. -
疾病相关
Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
序列相似性
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻译后修饰
Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation. -
细胞定位
Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. - Information by UniProt
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数据库链接
- Entrez Gene: 8864 Human
- Omim: 603426 Human
- SwissProt: O15055 Human
- Unigene: 58756 Human
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别名
- Circadian clock protein PERIOD 2 antibody
- FASPS antibody
- FASPS1 antibody
see all
图片
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Western blot - Anti-PER2 antibody (ab272930)All lanes : Anti-PER2 antibody (ab272930) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : PER2 transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated anti-rabbit IgG5% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272930 尚未被引用在任何文献中。