Anti-PER1抗体[2715C2] (ab58886)
![Western blot - Anti-PER1 antibody [2715C2] (ab58886)](https://www.abcam.cn/ps/products/58/ab58886/Images/ab58886.gif "Western blot - Anti-PER1 antibody [2715C2] (ab58886)")
Key features and details
- Mouse monoclonal [2715C2] to PER1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-PER1抗体[2715C2]
参阅全部 PER1 一抗 -
描述
小鼠单克隆抗体[2715C2] to PER1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment
预测可用于: Human
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免疫原
Recombinant fragment of Human PER1
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
2715C2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58886于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 136 kDa. Antibody has only been tested on the recombinant fragment (immunogen). We have no data regarding endogenous samples.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 136 kDa. Antibody has only been tested on the recombinant fragment (immunogen). We have no data regarding endogenous samples. |
靶标
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功能
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
NPAS2-BMAL1
BMAL2-induced transactivation. -
组织特异性
Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney. -
序列相似性
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻译后修饰
Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. -
细胞定位
Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. - Information by UniProt
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数据库链接
- Entrez Gene: 5187 Human
- Omim: 602260 Human
- SwissProt: O15534 Human
- Unigene: 445534 Human
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别名
- Circadian clock protein PERIOD 1 antibody
- Circadian clock protein PERIOD1 antibody
- Circadian pacemaker protein Rigui antibody
see all
图片
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Western blot - Anti-PER1 antibody [2715C2] (ab58886)Anti-PER1 antibody [2715C2] (ab58886) + immunising recombinant fragment
Predicted band size: 136 kDa
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
数据表及文件
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Datasheet download
文献 (0)
ab58886 尚未被引用在任何文献中。
