Anti-Peptide YY/PYY抗体(ab22663)
Key features and details
- Rabbit polyclonal to Peptide YY/PYY
- Suitable for: IHC-FoFr, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-Peptide YY/PYY抗体
参阅全部 Peptide YY/PYY 一抗 -
描述
兔多克隆抗体to多肽YY/PYY -
宿主
Rabbit -
特异性
Immunostaining may be abolished by preincubation with 1nmol synthetic porcine PYY per mL diluted antibody. NPY and PP (10nmol/mL) have no effect on immunostaining. -
经测试应用
适用于: IHC-FoFr, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Rabbit, Dog, Human
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免疫原
Full length native protein (purified) corresponding to Pig Peptide YY/PYY aa 1-36.
Sequence:YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY
Database link: P68005 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.07% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22663于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr |
Use at an assay dependent concentration.
|
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| IHC-P | (4) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-FoFr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. -
序列相似性
Belongs to the NPY family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 607156 Dog
- Entrez Gene: 5697 Human
- Entrez Gene: 217212 Mouse
- Entrez Gene: 287730 Rat
- Omim: 600781 Human
- SwissProt: P68004 Dog
- SwissProt: P10082 Human
- SwissProt: Q9EPS2 Mouse
see all -
别名
- GHYY antibody
- MGC19143 antibody
- Peptide tyrosine tyrosine antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)Image courtesy of Carl Hobbs, Kings College London, U.K.
IHC image of Peptide YY/PYY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)Image courtesy of Carl Hobbs, Kings College London, U.K.IHC image of Peptide YY/PYY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Peptide YY/PYY antibody (ab22663)Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)Image from Acosta A et al., PLoS One. 2011;6(10):e26137. Epub 2011 Oct 10. Fig 2.; doi:10.1371/journal.pone.0026137; October 10, 2011, PLoS ONE 6(10): e26137.ab22663 staining Peptide YY/PYY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue (left, WT; right, Peptide YY/PYY KO) was fixed with paraformaldehyde and blocked with 3% H2O2 in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).
数据表及文件
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Datasheet download
文献 (22)
ab22663 被引用在 22 文献中.
- Sridhar A et al. PYY (3-36) protects against high fat feeding induced changes of pancreatic islet and intestinal hormone content and morphometry. Biochim Biophys Acta Gen Subj 1867:130359 (2023). PubMed: 37001706
- Sridhar A et al. RYGB surgery has modest effects on intestinal morphology and gut hormone populations in the bypassed biliopancreatic limb but causes reciprocal changes in GLP-2 and PYY in the alimentary limb. PLoS One 18:e0286062 (2023). PubMed: 37228045
- Kim KS et al. Vertical sleeve gastrectomy induces enteroendocrine cell differentiation of intestinal stem cells through bile acid signaling. JCI Insight 7:N/A (2022). PubMed: 35503251
- Chu L et al. In vivo drug discovery for increasing incretin-expressing cells identifies DYRK inhibitors that reinforce the enteroendocrine system. Cell Chem Biol 29:1368-1380.e5 (2022). PubMed: 35998625
- Sridhar A et al. Differential effects of RYGB surgery and best medical treatment for obesity-diabetes on intestinal and islet adaptations in obese-diabetic ZDSD rats. PLoS One 17:e0274788 (2022). PubMed: 36137097
