Anti-Peptide YY/PYY抗体(ab131246)
Key features and details
- Rabbit polyclonal to Peptide YY/PYY
- Suitable for: IHC-P
- Reacts with: Rat, Human, Common marmoset
- Isotype: IgG
概述
-
产品名称
Anti-Peptide YY/PYY抗体
参阅全部 Peptide YY/PYY 一抗 -
描述
兔多克隆抗体to多肽YY/PYY -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat, Human, Common marmoset
预测可用于: Mouse, Dog, Pig, Macaque monkey, Chinese hamster
-
免疫原
-
阳性对照
- This antibody gave a positive signal in Rat normal Pancreas formalin fixed paraffin embedded tissue section.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131246于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (5) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. -
序列相似性
Belongs to the NPY family. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 607156 Dog
- Entrez Gene: 5697 Human
- Entrez Gene: 217212 Mouse
- Entrez Gene: 445018 Pig
- Entrez Gene: 287730 Rat
- Omim: 600781 Human
- SwissProt: P68004 Dog
- SwissProt: P10082 Human
see all -
别名
- GHYY antibody
- MGC19143 antibody
- Peptide tyrosine tyrosine antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)Image courtesy of Carl Hobbs, Kings College London, U.K.
IHC image of Peptide YY/PYY staining in mouse pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/6000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)Image courtesy of Carl Hobbs, Kings College London, U.K.IHC image of Peptide YY/PYY staining in Human colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/8000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)Image Courtesy of Carl Hobbs, Kings College London, U.K.IHC image of Peptide YY/PYY staining in marmoset Jejunum formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/5000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)IHC image of Peptide YY/PYY staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131246, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab131246 被引用在 3 文献中.
- Kim J et al. Clinicopathologic Impact of Peptide Hormonal Expression in Rectal Neuroendocrine Tumors. Arch Pathol Lab Med N/A:N/A (2022). PubMed: 36191342
- Rodnoi P et al. Neuropeptide Y expression marks partially differentiated ß cells in mice and humans. JCI Insight 2:N/A (2017). ICC/IF ; Mouse . PubMed: 28614797
- Suzuki H & Yamamoto T Amylin-like immunoreactivity in pancreatic X cells of the black-spotted frog Rana (Pelophylax) nigromaculata. Tissue Cell 46:535-9 (2014). IHC-Fr ; Frog . PubMed: 25458814