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重组RabMAb

重组Anti-Pentraxin 3/PTX3抗体[EPR18678-105] - BSA and Azide free (ab226866)

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Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
  • Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
  • Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18678-105] to Pentraxin 3/PTX3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Pentraxin 3/PTX3抗体[EPR18678-105] - BSA and Azide free
    参阅全部 Pentraxin 3/PTX3 一抗

  • 描述

    兔单克隆抗体[EPR18678-105] to Pentraxin 3/PTX3 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IP, ICC/IF, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: HUVEC cells.

  • 常规说明

    ab226866 is the carrier-free version of ab190838.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226866于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能

    Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

  • 序列相似性

    Contains 1 pentaxin domain.

  • 翻译后修饰

    Glycosylated.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AI607804 antibody
      • Pentaxin 3 antibody
      • Pentaxin-related protein PTX3 antibody
      • Pentraxin 3 long antibody
      • Pentraxin-related gene antibody
      • Pentraxin-related gene rapidly induced by IL-1 beta antibody
      • Pentraxin-related protein PTX3 antibody
      • PTX3 antibody
      • PTX3_HUMAN antibody
      • TNF alpha-induced protein 5 antibody
      • TNFAIP5 antibody
      • TSG-14 antibody
      • TSG14 antibody
      • Tumor necrosis factor alpha-induced protein 5 antibody
      • Tumor necrosis factor-inducible gene 14 protein antibody
      • Tumor necrosis factor-inducible protein TSG-14 antibody
      see all

    图片

    • Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
      Immunoprecipitation - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)

      Pentraxin 3/PTX3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.


      Lane 1: HUVEC whole cell lysate 10 μg (Input).
      Lane 2: ab190838 IP in HUVEC whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

    • Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
      Flow Cytometry (Intracellular) - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
      Immunocytochemistry/ Immunofluorescence - Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3/PTX3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).


      -ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190838).

    • Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)
      Anti-Pentraxin 3/PTX3 antibody [EPR18678-105] - BSA and Azide free (ab226866)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226866？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226866 尚未被引用在任何文献中。

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