AB88673

Anti-PELO抗体

Name: PELO抗体 (ab88673)| Abcam中文官网
Brand: Abcam Limited
SKU: AB88673
Price: 4606 CNY
Availability: InStock

Anti-PELO antibody

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(1 Publication)

Mouse Polyclonal PELO antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PELO.

查看别名

CGI-17, PELO, Protein pelota homolog, hPelota, Protein Dom34 homolog

3 Images

Unknown

Western blot - Anti-PELO antibody (AB88673)

All lanes:

Western blot - Anti-PELO antibody (ab88673) at 1 µg/mL

All lanes:

Hela cell lysate at 50 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Unknown

Western blot - Anti-PELO antibody (AB88673)

All lanes:

Western blot - Anti-PELO antibody (ab88673) at 1 µg/mL

All lanes:

Human stomach lysate at 50 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Unknown

Western blot - Anti-PELO antibody (AB88673)

All lanes:

Western blot - Anti-PELO antibody (ab88673) at 1 µg/mL

Lane 1:

PELO transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

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关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

Recombinant Full Length Protein corresponding to Human PELO.

Q9BRX2

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PELO also known as Pelota Homolog is a protein with a molecular mass of approximately 44 kDa. It acts as a ribosome-associated protein involved in no-go decay (NGD) a quality control mechanism that resolves stalled ribosomes. PELO helps target and degrade mRNA whose translation is impaired. You can find expression of PELO in various human tissues with predominant activity observed in the heart liver and skeletal muscle.

Biological function summary

PELO contributes to the maintenance of cellular homeostasis by ensuring proper protein synthesis. It acts as part of a surveillance complex that detects and rectifies errors in mRNA translation. This function associates PELO with other factors like HBS1L which help facilitate ribosomal recycling under stress or erroneous conditions. This modulatory role is critical in mitigating potentially harmful effects of translation errors on cellular function.

Pathways

PELO operates within the major ribosome quality control pathway focusing on detecting stalled ribosomes and reclaiming their components for future use. This pathway links PELO with proteins such as HBS1L and ABCE1. These interactions highlight the fine regulatory mechanisms that exist to preserve fidelity and efficiency of translation processes in cells.

PELO exhibits connections with genetic translation-related abnormalities and neurodegenerative disorders. Abnormalities in PELO function correlate with diseases like Pelizaeus-Merzbacher disease which impacts myelin formation in the central nervous system. The connection of PELO with other proteins involved in translation fidelity highlights its potential influence in disorders related to protein synthesis errors.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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Visit the Troubleshooting

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靶点信息

Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed : 21448132, PubMed : 23667253, PubMed : 27543824, PubMed : 27863242). In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel (PubMed : 27543824, PubMed : 27863242). Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway (PubMed : 21448132, PubMed : 32006463). As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy (PubMed : 29861391).

See full target information PELO

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 188:2545-55 PubMed22331067

2012

IκB kinase β is required for activation of NF-κB and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Lupino,Cristina Ramondetti,Marco Piccinini

View all publications

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Anti-PELO抗体

Anti-PELO antibody

Western blot - Anti-PELO antibody (AB88673)

Western blot - Anti-PELO antibody (AB88673)

Western blot - Anti-PELO antibody (AB88673)