AB288612

重组Anti-PEG10/EDR抗体[YCA-R20051-16-1 H3L3] - BSA and Azide free (Capture)

Anti-PEG10/EDR antibody [YCA-R20051-16-1 H3L3] - BSA and Azide free (Capture)

Recombinant
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Rabbit Recombinant Monoclonal PEG10/EDR antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

EDR, KIAA1051e, MAR2, MART2, MEF3L1, RGAG3, PEG10, Retrotransposon-derived protein PEG10, Embryonal carcinoma differentiation-regulated protein, Mammalian retrotransposon-derived protein 2, Myelin expression factor 3-like protein 1, Paternally expressed gene 10 protein, Retrotransposon gag domain-containing protein 3, Retrotransposon-derived gag-like polyprotein, Ty3/Gypsy-like protein, MEF3-like protein 1

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Sandwich ELISA - Anti-PEG10/EDR antibody [YCA-R20051-16-1 H3L3] - BSA and Azide free (Capture) (AB288612)

sELISA

Lab

Sandwich ELISA - Anti-PEG10/EDR antibody [YCA-R20051-16-1 H3L3] - BSA and Azide free (Capture) (AB288612)

Sandwich ELISA of ab288650 with the capture antibody ab288612 dilution at 2 µg/mL and detector antibody ab288628 dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

YCA-R20051-16-1 H3L3

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab288612 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human PEG10/EDR. The recommended pair for sandwich ELISA is:
Capture: ab288612, Human PEG10/EDR Capture Antibody (unconjugated)
Detector: ab288628, Human PEG10/EDR Detector Antibody (unconjugated)
The reference range value is 1.56-100 ng/mL.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PEG10 also known as EDR2 is a paternally expressed imprinted gene that encodes the PEG10 protein. The PEG10 protein has a molecular mass of approximately 150 kDa. It shows expression most notably in the placenta during development but also in other tissues like the liver and brain. The protein contains two open reading frames that produce different products via a frameshift mechanism similar to retroviral proteins.

Biological function summary

The PEG10 protein plays multiple roles especially in cell proliferation apoptosis inhibition and promoting cell survival. It can be part of larger protein complexes that modulate cellular responses to stress and developmental cues. The protein's multifaceted activities are important during embryogenesis where it supports trophoblast development and placental formation.

Pathways

PEG10 interacts with signaling cascades such as the TGF-beta signaling pathway and is involved in cell cycle regulation. PEG10 works alongside proteins like paternally expressed genes that connect with TGF-beta pathways influencing cellular differentiation and growth. Its integration in these pathways highlights its role in managing cellular environments and responses to cellular stress signals.

PEG10 expression over-activation has associations with cancer especially hepatocellular carcinoma and pancreatic cancer. In these contexts PEG10 interacts with proteins like H3L3 contributing to oncogenic pathways that drive tumor cell proliferation and survival. Elevated levels of PEG10 can serve as potential biomarkers for certain cancers suggesting their role in disease progression and possible therapeutic targeting.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Retrotransposon-derived protein that binds its own mRNA and self-assembles into virion-like capsids (PubMed : 34413232). Forms virion-like extracellular vesicles that encapsulate their own mRNA and are released from cells, enabling intercellular transfer of PEG10 mRNA (PubMed : 34413232). Binds its own mRNA in the 5'-UTR region, in the region near the boundary between the nucleocapsid (NC) and protease (PRO) coding sequences and in the beginning of the 3'-UTR region (PubMed : 34413232). Involved in placenta formation : required for trophoblast stem cells differentiation (By similarity). Involved at the immediate early stage of adipocyte differentiation (By similarity). Overexpressed in many cancers and enhances tumor progression : promotes cell proliferation by driving cell cycle progression from G0/G1 (PubMed : 12810624, PubMed : 16423995, PubMed : 26235627, PubMed : 28193232). Enhances cancer progression by inhibiting the TGF-beta signaling, possibly via interaction with the TGF-beta receptor ACVRL1 (PubMed : 15611116, PubMed : 26235627, PubMed : 30094509). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter; additional evidences are however required to confirm this result (By similarity).

See full target information PEG10

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PEG10/EDR抗体[YCA-R20051-16-1 H3L3] - BSA and Azide free (Capture)

Anti-PEG10/EDR antibody [YCA-R20051-16-1 H3L3] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-PEG10/EDR antibody [YCA-R20051-16-1 H3L3] - BSA and Azide free (Capture) (AB288612)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原