PE/Cy5® Anti-CD11b抗体[M1/70] (ab25533)
![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.cn/ps/products/25/ab25533/Images/ab25533-276448-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.png "Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)")
Key features and details
- PE/Cy5® Rat monoclonal [M1/70] to CD11b
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy5®. Ex: 496nm, Em: 670nm
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
PE/Cy5® Anti-CD11b抗体[M1/70]
参阅全部 CD11b 一抗 -
描述
PE/Cy5®大鼠单克隆抗体[M1/70] to CD11b -
宿主
Rat -
偶联物
PE/Cy5®. Ex: 496nm, Em: 670nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.
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阳性对照
- Mouse spleen cells.
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常规说明
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
M1/70 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
- Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
- PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
- PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
- Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
- violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
- FITC Anti-CD11b antibody [M1/70] (ab24874)
- PE Anti-CD11b antibody [M1/70] (ab25175)
- redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
- APC Anti-CD11b antibody [M1/70] (ab25482)
- APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
- Anti-CD11b antibody [M1/70] (ab8878)
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25533于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | (11) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
组织特异性
Predominantly expressed in monocytes and granulocytes. -
疾病相关
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 16409 Mouse
- SwissProt: P05555 Mouse
- Unigene: 262106 Mouse
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别名
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
图片
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Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 µg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 µg (dashed line).
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![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.cn/ps/products/25/ab25533/Images/ab25533-36039-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.jpg)
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)This image is courtesy of an anonymous Abreviewab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils. -
![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.cn/ps/products/25/ab25533/Images/ab25533-36038-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.jpg)
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions. -
![Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)](https://www.abcam.cn/ps/products/25/ab25533/Images/ab25533-36037-anti-cd11b-antibody-m1-70-pe-cy5-flow-cytometry.jpg)
Flow Cytometry - PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)This image is courtesy of an anonymous Abreviewab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy: Live Macrophages.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab25533 被引用在 9 文献中.
- Dai X et al. Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization. Bioact Mater 6:2029-2038 (2021). PubMed: 33474514
- Liu Q et al. Melatonin alleviates morphine analgesic tolerance in mice by decreasing NLRP3 inflammasome activation. Redox Biol 34:101560 (2020). PubMed: 32413745
- Wen G et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). PubMed: 29437605
- Xu J et al. Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation of RhoA expression. Acta Biomater 72:434-446 (2018). Flow Cyt . PubMed: 29649639
- Kotla S et al. Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor expression for 15(S)-hydroxyeicosatetraenoic acid-induced monocyte trafficking. J Biol Chem 292:14885-14901 (2017). PubMed: 28724635
- Chen X et al. Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut. Sci Rep 6:24152 (2016). Flow Cyt . PubMed: 27080036
- Zeng SL et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). PubMed: 25936350
- Vrecenak JD et al. Stable long-term mixed chimerism achieved in a canine model of allogeneic in utero hematopoietic cell transplantation. Blood 124:1987-95 (2014). PubMed: 24869940
- Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . PubMed: 23806234