Anti-PECI/ECI2抗体(ab224441)
Key features and details
- Rabbit polyclonal to PECI/ECI2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PECI/ECI2抗体
参阅全部 PECI/ECI2 一抗 -
描述
兔多克隆抗体to PECI/ECI2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PECI/ECI2 aa 100-250.
Database link: O75521 -
阳性对照
- IHC-P: Human liver, kidney and duodenum tissue. WB: RT4 and U-251 MG sp cell lysates. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224441于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. -
组织特异性
Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells. -
序列相似性
In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
Contains 1 ACB (acyl-CoA-binding) domain. -
细胞定位
Mitochondrion and Peroxisome matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 10455 Human
- Omim: 608024 Human
- SwissProt: O75521 Human
- Unigene: 15250 Human
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别名
- 2-trans-enoyl-CoA isomerase antibody
- ACBD2 antibody
- acyl Coenzyme A binding domain containing 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PECI/ECI2 antibody (ab224441)
Paraffin-embedded human duodenum tissue stained for PECI/ECI2 using ab224441 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PECI/ECI2 antibody (ab224441)Paraffin-embedded human kidney tissue stained for PECI/ECI2 using ab224441 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PECI/ECI2 antibody (ab224441)Paraffin-embedded human liver tissue stained for PECI/ECI2 using ab224441 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-PECI/ECI2 antibody (ab224441)All lanes : Anti-PECI/ECI2 antibody (ab224441) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Predicted band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PECI/ECI2 antibody (ab224441)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PECI/ECI2 (green) using ab224441 at 4 µg/ml in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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