PE Anti-VCAM1 antibody [EPR27027-79]

• Flow Cyt

Lab

Flow Cytometry - PE Anti-VCAM1 antibody [EPR27027-79] (AB323936)

Flow cytometry overlay histogram showing left Hut-78 positive cells and right negative SK-OV-3 stained with ab323936 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactionfollowed by the antibody (ab323936) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt

Lab

Flow Cytometry - PE Anti-VCAM1 antibody [EPR27027-79] (AB323936)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab323936 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab323936 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live cells.