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AB225342

重组PE Anti-TOMM20抗体[EPR15581-54]

PE Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

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(1 Publication)

Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20

1 Images
Flow Cytometry (Intracellular) - PE Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB225342)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB225342)

Overlay histogram showing HeLa cells stained with ab225342 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225342, 1/1000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

不同偶联物与剂型 (10)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 660 APC

    APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • HRP

    HRP Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • Unconjugated

    Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

  • Carrier free

    Anti-TOMM20 antibody [EPR15581-54] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15581-54

亚型

IgG

偶联物

PE

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Do Not Freeze|Store in the dark

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
See full target information TOMM20

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37370835

2023

Enhancement of the Anticancer Ability of Natural Killer Cells through Allogeneic Mitochondrial Transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-Hoon Kim,Mi-Jin Kim,Mina Lim,Jihye Kim,Hyunmin Kim,Chang-Koo Yun,Yun-Joo Yoo,Youngjun Lee,Kyunghoon Min,Yong-Soo Choi
View all publications

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

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