Key features and details
- PE Mouse monoclonal [40C1285.6] to TLR3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
参阅全部 TLR3 一抗
描述PE小鼠单克隆抗体[40C1285.6] to TLR3
偶联物PE. Ex: 488nm, Em: 575nm
经测试应用适用于: Flow Cyt (Intra)more details
This antibody was developed against KLH-conjugated synthetic peptide of human TLR3.
- Human intestine or Ramos. Flow Cyt (Intra): Ramos, A549 cells
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存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Protein G purified
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|Flow Cyt (Intra)|
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 104 kDa.
This antibody has tested negative by western blot in mouse intestine cell lysate.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
组织特异性Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
疾病相关Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
序列相似性Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain.
结构域ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
翻译后修饰Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
细胞定位Endoplasmic reticulum membrane. Endosome membrane.
- Information by UniProt
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
ab45093 staining TLR3 in Human A549 (lung epithelial) cell line by Flow Cytometry. The sample was incubated with the primary antibody (5μg/ml in 0.1% Saponin + 1% FCS in PBS) for 45 minutes at 4°C. 1 = isotype control, 2 = ab45093
ab45093, at 3 ug/ 1X10^6 cells, staining Ramos cells by Intracellular flow cytometric analysis. Toll-Like-Receptor3 (open peak) and isotype negative control (shaded peak).
ab45093 被引用在 1 文献中.
- Franzen-Röhl E et al. Increased cell-mediated immune responses in patients with recurrent herpes simplex virus type 2 meningitis. Clin Vaccine Immunol 18:655-60 (2011). Flow Cyt . PubMed: 21325490