重组PE Anti-Smad2 (phospho T8) + Smad3 (phospho T8)抗体[EPR23682-64] (ab318411)
![PE Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64] (ab318411)](https://www.abcam.cn/ps/products/318/ab318411/Images/ab318411-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "PE Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64] (ab318411)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR23682-64] to Smad2 (phospho T8) + Smad3 (phospho T8)
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: PE. Ex: 488nm, Em: 575nm
概述
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产品名称
PE Anti-Smad2 (phospho T8) + Smad3 (phospho T8)抗体[EPR23682-64] -
描述
PE兔单克隆抗体[EPR23682-64] to Smad2 (phospho T8) + Smad3 (phospho T8) -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Target binding affinity, Antibody labellingmore details -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
常规说明
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23682-64 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318411于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
|
| Antibody labelling |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
靶标
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细胞定位
Smad2: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Smad3: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).
图片
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PE Anti-Smad2 (phospho T8) + Smad3 (phospho T8) antibody [EPR23682-64] (ab318411)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab318411 尚未被引用在任何文献中。