重组PE Anti-ROCK2 + ROCK1抗体[EP786Y] (ab224972)
![Flow Cytometry (Intracellular) - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)](https://www.abcam.cn/ps/products/224/ab224972/Images/ab224972-315863-anti-rock1-antibody-ep786y-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP786Y] to ROCK2 + ROCK1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-ROCK2 + ROCK1抗体[EP786Y] -
描述
PE兔单克隆抗体[EP786Y] to ROCK2 + ROCK1 -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
This antibody recognizes both the cleaved C-terminus of ROCK 1 (30 kDa) and full length protein (158 kDa). The immunogen used for this product shares 83% homology with ROCK2.
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经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP786Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224972于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
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| WB |
1/20000.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/1000. |
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WB
1/20000. |
靶标
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细胞定位
ROCK2: Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. ROCK1: Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes. -
数据库链接
- Entrez Gene: 6093 Human
- Entrez Gene: 9475 Human
- Entrez Gene: 19877 Mouse
- Entrez Gene: 19878 Mouse
- Entrez Gene: 25537 Rat
- Entrez Gene: 81762 Rat
- Omim: 601702 Human
- Omim: 604002 Human
see all
图片
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Flow Cytometry (Intracellular) - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
Overlay histogram showing HeLa cells stained with ab224972 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224972, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)](https://www.abcam.cn/ps/products/224/ab224972/Images/ab224972-445055-anti-rock2-rock1-antibody-ep786y-western-blot-recombinant-human.jpg)
Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)All lanes : Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/1000 dilution
Lane 1 : Recombinant Human ROCK1 protein (aa 1114 to 1354) (30 kDa)
Lane 2 : Recombinant Human ROCK2 protein (aa 1132 to 1388) (30 kDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab45171, the same antibody clone in a different buffer formulation.
Loading control: Anti-6X His tag® antibody [EPR20547] (ab213204)
Blocking buffer and concentration: 5% NFDM/TBST
Exposure Times:
Lane 1: 3 seconds
Lane 2: 7 seconds
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![PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)](https://www.abcam.cn/ps/products/224/ab224972/Images/ab224972-2-benefits-of-recombinant-antibodies.png)
PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab224972)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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