AB209478

重组PE兔IgG,单克隆抗体[EPR25A] -同型对照

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(10 Publications)

Anti-Rabbit IgG, monoclonal [EPR25A] - Isotype Control- PE conjugated (ab209478) is a rabbit recombinant monoclonal antibody used in Flow Cytometry (Intra), Flow Cytometry, ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

40 Images

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab316176 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316176 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD11b.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left Hela positive cells and right negative Jurkat stained with ab313946 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab313946) (1x 10⁶ in 100μl at 0.04 μg/ml (1/12500)) for 30 min on ice.

Isotype control antibody (black line) was PE Mouse IgG1 [B11/6] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left K-562 positive cells and right negative PC3 stained with ab314270 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314270) (1x 10⁶ in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in K-562 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing HeLa cells stained with ab316178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab316178) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab313951 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab313951 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Events were gated on viable CD56(-) lymphocytes.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left HeLa positive cells and right negative THP-1 stained with ab314417 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314417) (1x 10⁶ in 100μl at 1.0 μg/ml (1/500)) for 30min on ice.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314292 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314292 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD16.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on Viable Cells.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314947 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314947 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶in 100 μl at 0.04 μg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD14.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing HeLa cells stained with ab314253 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314253) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left Raji positive cells and right negative A-375 stained with ab314248 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314248) (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in Raji Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 1%FBS for 3hours then 100nM Calyculin A for 10min (Green) / Untreated control (Magenta) cells labelling IkappaB-alpha (pS36) with ab325262 at 1/5000 dilution (0.01ug) / Green and Magenta compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) and (Grey) isotype control.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling EBV Induced Gene 2/EBI2 with ab325228 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

Gated on viable cell.
Cells were co-stained with CD19 conjugated to Alexa Fluor®647.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a human SLC38A5 expression vector containing a Myc tag (Middle) / 293T cells transfected with an empty expression vector containing a Myc tag (Right) cells labelling SLC38A5 / SNAT5 with ab324287 at 1/50000 dilution (0.001ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

Live cells were surface stained with our antibody, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of LNCaP (human prostate carcinoma epithelial cell, Left) / PC-3 (human prostate adenocarcinoma epithelial cell, Right) cells labelling LDL Receptor with ab325118 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Low expression : LNCaP.
Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of 293T (human embryonic kidney) cells transfected with human TNFRSF9 labeling GFP stained with ab303588 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype control (left). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab303588) (1x 10⁶ in 100 µl at 1 µg/ml (1/500)) for 30 min at 22°C.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 585/42 bandpass filter.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left THP-1 positive cells and right negative HCT116 stained with ab314261 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314261) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min at 22°C.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Overlay histogram showing HeLa cells untreated (magenta line) and HeLa cells treated with Chloroquine, 50μM, 24 hours, (green line) stained with ab225454. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225454, 1μg/ml) for 30 min at 22°C.

Isotype control antibody (HeLa cells untreated - black line, HeLa cells treated - grey line) was Rabbit IgG (monoclonal) Phycoerythrin (PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab209478) used at the same concentration and conditions as the primary antibody.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labelling LDL Receptor with ab325118 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Low expression : HEK-293.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Gated on viable cell.
Cells were co-stained with CD56 conjugated to Brilliant Violet 421.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T cells transfected with a mouse CCR4 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling CCR4 with ab324333 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were surface stained with isotype control or our antibody. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left THP-1 positive cells and right negative Jurkat stained with ab314295 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314295) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314260 (right) or PE Mouse IgG1 [B11/6] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314260 or PE Mouse IgG1 [B11/6] - Isotype Control (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab307635 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab307635) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (100 μl at 0.008μg/ml (1/62500)) for 30 min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live single cells.

Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / KARPAS-299(human T cell lymphoma cell, Right) cells labelling EBV Induced Gene 2/EBI2 with ab325228 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cell.
Negative control : HeLa.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left A-375 positive cells and right negative MCF7 stained with ab314251 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314251) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left U87-MG positive cells and right negative HUVEC stained with ab314406 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314406) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab307640 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab307640) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (100 μl at 0.04μg/ml (1/12500)) for 30 min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live lymphocytes.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing wild-type A-549 (green line) and SLC2A1 knockout A-549 stained with ab209449 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab209449) (1x 10⁶ in 100μl at 0.008 μg/ml (1/62500)) for 30min at 22°C.

Isotype control antibody was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) in A-549 WT cells (black line) and A-549-SLC2A1 KO cells (grey line), used at the same concentration and conditions as the primary antibody.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing HeLa cells stained with ab314402 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314402) (1x 10⁶ in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a mouse CD27 expression vector (Middle) / 293T cells transfected with an empty expression vector (Right) cells labelling CD27 with ab324175 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing wild-type HeLa (green line) and L1CAM knockout HeLa stained with ab315143 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab315143) (1x 10⁶in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line, L1CAM knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing U-87 positive cells (left) and A549 negative cells (right) stained with ab325094 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab325094) (1x 10⁶ cells in 100μl at 0.2 μg/ml (1/2,500)) for 30min on ice.

Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (black line) was used at the same concentration and conditions as the primary antibody (red line). Unlabelled sample was also used as a control (blue line).

Acquisition of > 10,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) (top) or human peripheral blood mononuclear cells (PBMCs) treated with CD3/CD28 activation for 72h (bottom), with ab307641 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab307641) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (1x 106 in 100μl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD8.

不同偶联物与剂型 (12)

519 FITC

FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
660 APC

APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
675 PerCP

PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
603 Alexa Fluor® 568

Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
617 Alexa Fluor® 594

Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
519 Alexa Fluor® 488

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
HRP

HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
665 Alexa Fluor® 647

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
421 Alexa Fluor® 405

Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
565 Alexa Fluor® 555

Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Biotin

Biotin Isotype Control [EPR25A]
775 Alexa Fluor® 750

Alexa Fluor® 750 Rabbit IgG, monoclonal [EPR25A] - Isotype Control

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25A

亚型

IgG

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

应用

ICC/IF, Flow Cyt (Intra)

applications

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used." } } }

style

left-column

产品详情

What is this antibody validated in?
PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF).

Other related products
We have a range of other formats of antibody clone [EPR25A] also available for your convenience: ab172730, Alexa Fluor^® 488 - ab199091, Alexa Fluor^® 647 - ab199093, Carrier free - ab199376, HRP - ab199507, Alexa Fluor^® 405 - ab208150, Alexa Fluor^® 594 - ab208568, Alexa Fluor^® 555 - ab208569, PE - ab209478, Alexa Fluor^® 568 - ab209613, Carrier free - ab210849, PerCP - ab222107, FITC - ab223339, APC - ab232814, Biotin - ab320073, Alexa Fluor^® 750 - ab322086

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Store in the dark

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

style

left-column

文献 (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112894 PubMed40678547

2025

Deep metabolic profiling of immune cells by spectral flow cytometry-A comprehensive validation approach.

Applications

Unspecified application

Species

Unspecified reactive species

Claire L Wishart,Alanna G Spiteri,Jian Tan,Claudio Counoupas,James A Triccas,Laurence Macia,Nicholas J C King

Frontiers in medicine 12:1583271 PubMed40421296

2025

Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Qianren Zheng,Jie Chen,Yixun Huang,Weikai Chen,Dandan Cheng,Qianqian Jia,Meiqin Zhu,Youguo Liao,Qiulin He,Shunli Wu

Journal for immunotherapy of cancer 12: PubMed39266214

2024

S100A9 and HMGB1 orchestrate MDSC-mediated immunosuppression in melanoma through TLR4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Feyza Gül Özbay Kurt,Beatrice-Ana Cicortas,Bianca M Balzasch,Carolina De la Torre,Volker Ast,Ece Tavukcuoglu,Cagla Ak,Sebastian A Wohlfeil,Adelheid Cerwenka,Jochen Utikal,Viktor Umansky

Molecular therapy : the journal of the American Society of Gene Therapy 32:2393-2405 PubMed38720457

2024

GLUT1 overexpression enhances CAR T cell metabolic fitness and anti-tumor efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhe Shi,Ivan S Kotchetkov,Anton Dobrin,Sophie A Hanina,Vinagolu K Rajasekhar,John H Healey,Michel Sadelain

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300812 PubMed37357136

2023

Engineered Biomimetic Fibrillar Fibronectin Matrices Regulate Cell Adhesion Initiation, Migration, and Proliferation via α5β1 Integrin and Syndecan-4 Crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Seungkuk Ahn,Upnishad Sharma,Krishna Chaitanya Kasuba,Nico Strohmeyer,Daniel J Müller

Journal for immunotherapy of cancer 11: PubMed36759012

2023

Pharmacological tumor PDL1 depletion with chlorambucil treats ovarian cancer and melanoma: improves antitumor immunity and renders anti-PDL1-resistant tumors anti-PDL1-sensitive through NK cell effects.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Bai,Alvaro S Padron,Yilun Deng,Yiji J Liao,Clare J Murray,Carlos Ontiveros,Suresh J Kari,Aravind Kancharla,Anand V R Kornepati,Myrna Garcia,Ryan Michael Reyes,Harshita B Gupta,Jose R Conejo-Garcia,Tyler Curiel

Frontiers in neurology 13:869103 PubMed35911883

2022

The Quest for Anti-α-Synuclein Antibody Specificity-Lessons Learnt From Flow Cytometry Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Leupold,Veronika Sigutova,Elizaveta Gerasimova,Martin Regensburger,Sebastian Zundler,Friederike Zunke,Wei Xiang,Beate Winner,Iryna Prots

Nature communications 12:2133 PubMed33837219

2021

IL-33 expression in response to SARS-CoV-2 correlates with seropositivity in COVID-19 convalescent individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Michal A Stanczak,David E Sanin,Petya Apostolova,Gabriele Nerz,Dimitrios Lampaki,Maike Hofmann,Daniel Steinmann,Marvin Krohn-Grimberghe,Robert Thimme,Gerhard Mittler,Cornelius F Waller,Edward J Pearce,Erika L Pearce

Aging 12:25845-25864 PubMed33234721

2020

The long noncoding RNA LINC01140/miR-140-5p/FGF9 axis modulates bladder cancer cell aggressiveness and macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Shuiqing Wu,Ran Xu,Xuan Zhu,Haiqing He,Jinhua Zhang,Qi Zeng,Yinhuai Wang,Xiaokun Zhao

Arteriosclerosis, thrombosis, and vascular biology 39:482-495 PubMed30626206

2019

Spermine on Endothelial Extracellular Vesicles Mediates Smoking-Induced Pulmonary Hypertension Partially Through Calcium-Sensing Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhu,Rui Xiao,Xiuyun Zhang,Yuheng Lang,Fangbo Liu,Zhe Yu,Jiwei Zhang,Yuan Su,Yankai Lu,Tao Wang,Shengquan Luo,Jian Wang,Ming-Lin Liu,Jocelyn Dupuis,Zhi-Cheng Jing,Tong Li,Weining Xiong,Qinghua Hu

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组PE兔IgG,单克隆抗体[EPR25A] -同型对照

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

不同偶联物与剂型 (12)

关键信息

宿主种属

克隆

克隆号

亚型

偶联物

激发波长/发射波长

不含载体蛋白

应用

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

产品实验方案

靶点信息

文献 (10)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies