重组PE兔IgG,单克隆抗体[EPR25A] -同型对照

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left Hela positive cells and right negative Jurkat stained with ab313946 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab313946) (1x 10⁶ in 100μl at 0.04 μg/ml (1/12500)) for 30 min on ice.

Isotype control antibody (black line) was PE Mouse IgG1 [B11/6] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab306214 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated for 30 min at 4°C in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab306214) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100μl at 0.00032 μg/ml (1/1562500)) for 30min at 4°C. The cells were simultaneously stained with CD14.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable monocytes.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab313951 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab313951 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

Events were gated on viable CD56(-) lymphocytes.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left HeLa positive cells and right negative THP-1 stained with ab314417 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314417) (1x 10⁶ in 100μl at 1.0 μg/ml (1/500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

• Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab323732 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were co-stained with anti human CD19 conjugated to Alexa Fluor^®647.
Gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left THP-1 positive cells and right negative Jurkat stained with ab314295 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314295) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing HeLa cells stained with ab314253 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314253) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left Raji positive cells and right negative A-375 stained with ab314248 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314248) (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in Raji Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling EBV Induced Gene 2/EBI2 with ab325228 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

Gated on viable cell.
Cells were co-stained with CD56 conjugated to Brilliant Violet 421.

• Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling EBV Induced Gene 2/EBI2 with ab325228 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

Gated on viable cell.
Cells were co-stained with CD19 conjugated to Alexa Fluor®647.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left HUVEC positive cells and right negative HEK293 cells stained with ab307642 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab307642) (1x 106 in 100μl at 1μg/ml (1/500)) for 30 min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labelling LDL Receptor with ab325118 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Low expression : HEK-293.
Gated on viable cells.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of Human PBMCs (top) or PBMCs treated with 1μg/ml LPS and 1μg/ml Brefeldin A for 18h (bottom), with ab317072 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab317072 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD14.

Acquisition of >30000 events were collected using a 50mW Yellow/Green laser (561nm) and 585/16 bandpass filter. Events were gated on viable cells.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing HeLa cells stained with ab316178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab316178) (1x 10⁶ in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab316176 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316176 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD11b.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab307635 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab307635) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (100 μl at 0.008μg/ml (1/62500)) for 30 min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live single cells.

• Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of LNCaP (human prostate carcinoma epithelial cell, Left) / PC-3 (human prostate adenocarcinoma epithelial cell, Right) cells labelling LDL Receptor with ab325118 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Low expression : LNCaP.
Gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left A-375 positive cells and right negative MCF7 stained with ab314251 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314251) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left U87-MG positive cells and right negative HUVEC stained with ab314406 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314406) (1x 10⁶ in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab316176 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab316176 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.008 μg/ml (1/62500)) for 30min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab307640 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab307640) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (100 μl at 0.04μg/ml (1/12500)) for 30 min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live lymphocytes.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) (top) or human peripheral blood mononuclear cells (PBMCs) treated with CD3/CD28 activation for 72h (bottom), with ab307641 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (left). PBMCs were fixed and permeabilised with BD Cytofix/Cytoperm™ for 20 min. PBMCs were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab307641) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype (1x 106 in 100μl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD8.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing wild-type A-549 (green line) and SLC2A1 knockout A-549 stained with ab209449 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab209449) (1x 10⁶ in 100μl at 0.008 μg/ml (1/62500)) for 30min at 22°C.

Isotype control antibody was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) in A-549 WT cells (black line) and A-549-SLC2A1 KO cells (grey line), used at the same concentration and conditions as the primary antibody.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing HeLa cells stained with ab314402 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314402) (1x 10⁶ in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in HeLa Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing U-87 positive cells (left) and A549 negative cells (right) stained with ab325094 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab325094) (1x 10⁶ cells in 100μl at 0.2 μg/ml (1/2,500)) for 30min on ice.

Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (black line) was used at the same concentration and conditions as the primary antibody (red line). Unlabelled sample was also used as a control (blue line).

Acquisition of > 10,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing wild-type HeLa (green line) and L1CAM knockout HeLa stained with ab315143 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab315143) (1x 10⁶in 100μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line, L1CAM knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / KARPAS-299(human T cell lymphoma cell, Right) cells labelling EBV Induced Gene 2/EBI2 with ab325228 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cell.
Negative control : HeLa.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314947 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314947 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶in 100 μl at 0.04 μg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD14.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of 293T (human embryonic kidney) cells transfected with human TNFRSF9 labeling GFP stained with ab303588 (right) or Rabbit IgG (monoclonal) Phycoerythrin (ab209478) isotype control (left). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab303588) (1x 10⁶ in 100 µl at 1 µg/ml (1/500)) for 30 min at 22°C.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561 nm) and 585/42 bandpass filter.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314260 (right) or PE Mouse IgG1 [B11/6] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314260 or PE Mouse IgG1 [B11/6] - Isotype Control (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314292 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314292 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD16.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on Viable Cells.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left K-562 positive cells and right negative PC3 stained with ab314270 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314270) (1x 10⁶ in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in K-562 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left THP-1 positive cells and right negative HCT116 stained with ab314261 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314261) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

• Flow Cyt

Supplier Data

Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

Flow cytometry overlay histogram showing left SK-N-SH positive cells and right negative A-498 stained with ab322987 (red line). The cells were fixed with 8% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab322987) (1x 10⁶ in 100 ul at 0.0016 ug/ml (1/312500)) for 30min at 22°C.

Isotype control antibody Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.