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PE-Rabbit IgG -同型对照(ab37407)

Q&A (4)References (3)

概述

  • 产品名称

    PE-Rabbit IgG -同型对照
    参阅全部 Rabbit 亚类对照

  • 偶联物

    PE. Ex: 488nm, Em: 575nm

  • 特异性

    This antibody has been selected to be an isotype control as it has no known specificity.

  • 经测试应用

    适用于: Flow Cyt, ELISAmore details

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • 存储溶液

    pH: 7.3
    Preservative: 0.09% Sodium azide
    Constituents: 16% Sucrose, PBS

    Also contains stabilising agent.
  • Concentration information loading...

  • 纯度

    IgG fraction

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

  • 别名

    • rabbit isotype control

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab37407于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 10µl for 106 cells.
ELISA
Use at an assay dependent dilution.
说明
Flow Cyt
Use 10µl for 106 cells.
ELISA
Use at an assay dependent dilution.

文献 (3)

发表研究结果有使用 ab37407？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab37407 被引用在 3 文献中.

  • Sun R  et al. Therapeutic targeting miR130b counteracts diffuse large B-cell lymphoma progression via OX40/OX40L-mediated interaction with Th17 cells. Signal Transduct Target Ther 7:80 (2022). PubMed: 35301282
  • Bunkholt Elstrand M  et al. Mammalian target of rapamycin is a biomarker of poor survival in metastatic serous ovarian carcinoma. Hum Pathol 41:794-804 (2010). PubMed: 20153512
  • Dong HP  et al. Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry. Nat Protoc 3:955-64 (2008). PubMed: 18546596

客户评价及客户问答

Q&A
提交问题

1-4 of 4 Q&A

Question



Ein Kunde möchte die Konzentration wissen, wenn Sie Artikel Nr. ab37407 und ab72331 zusammen benutzt!



Danke DIr

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Answer

Vielen Dank für Ihre Anfrage.
Ich schlage vor die optimale Verdünnung für ab72331 zuerst zu testen und dann die Isotyp- Kontrolle (ab37407) in derselben Konzentration einzusetzen.
Die Konzentration von ab37407 ist Lot abhängig. Liegt aber meistens in etwa bei 0.1mg/ml. Bitte teilen Sie mir das Lot mit und ich kann Ihnen eine genauere Auskunft geben.
ab72331 hat eine Konzentration von 0.5mg/ml.
Ich hoffe diese Auskunft ist hilfreich. Bitte zögern Sie nicht mich nochmals mit der Lot -nummer zu kontaktieren.

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Question

Dear Sir or Madam,
Could you please tell me the concentration and volume of product
ab37407, Rabbit IgG Phycoerythrin Isotype Control ?
Thank you and best regards,

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Answer

Thank you for your inquiry.
We offer ab37407 as 100 tests. We recommend to use 10ul for one test (106 cells).
I can confirm that this product has a volume of 1ml.
The concentration of ab37407 is lot dependent. The lot that is in stock at the moment has a concentration of 0.01mg/mL.
I hope this information is helpful. Please do not hesitate to contact me again with any further questions or the lot number that you received.

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Question

Please send the concentrations of ab37406, lot GR45440-2 ab37407, lot GR44588-1.

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Answer

Thank you for contacting us. The IgG concentrations are: ab37406, lot GR45440-2: 0.1 mg/ml ab37407, lot GR44588-1: 0.01 mg/ml I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I am looking for an isotype control for Anti-Hsp90 beta antibody (Phycoerythrin) (ab115641). Is the following product of ab37407 the right one? https://www.abcam.com/Rabbit-IgG-Phycoerythrin-Isotype-Control-ab37407.html

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Answer

Thank you for your enquiry. The antibody ab37407 will be a suitable isotype control but please note that the stock concentration of the current batch of the control is 0.01 mg/ml, compared to 1.0 mg/ml for ab115641. To match the working concentrations of IgG you will need to dilute the HSP90 antibody stock solution 100-fold compared to the control. If you are working with live cells, consider that the amount of azide in each working solution will differ. Please let me know if you have any questions.

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