PE Anti-Perforin抗体[B-D48] (ab47226)
Key features and details
- PE Mouse monoclonal [B-D48] to Perforin
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
PE Anti-Perforin抗体[B-D48]
参阅全部 Perforin 一抗 -
描述
PE小鼠单克隆抗体[B-D48] to Perforin -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
Recognizes human Perforin, a 70 kDa protein.
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经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Perforin.
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阳性对照
- Flow Cyt (Intra): YT-Indy cell line
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常规说明
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 1 ml deionised water. -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 94.91% PBS, 5% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
B-D48 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47226于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
Use 10 µl to label 106 cells or 100 µl of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. Use 10 µl to label 106 cells or 100 µl of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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靶标
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功能
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. -
疾病相关
Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found. -
序列相似性
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 C2 domain.
Contains 1 EGF-like domain.
Contains 1 MACPF domain. -
结构域
The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands. -
翻译后修饰
N-glycosylated. -
细胞定位
Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 5551 Human
- Omim: 170280 Human
- SwissProt: P14222 Human
- Unigene: 2200 Human
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别名
- Cytolysin antibody
- FLH2 antibody
- HPLH2 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab47226 被引用在 12 文献中.
- Herrera FG et al. 50-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial. Int J Radiat Oncol Biol Phys 103:320-334 (2019). PubMed: 30267761
- Lam JKP et al. Emergence of CD4+ and CD8+ Polyfunctional T Cell Responses Against Immunodominant Lytic and Latent EBV Antigens in Children With Primary EBV Infection. Front Microbiol 9:416 (2018). Flow Cyt ; Human . PubMed: 29599759
- Pérez-Antón E et al. Impact of benznidazole treatment on the functional response of Trypanosoma cruzi antigen-specific CD4+CD8+ T cells in chronic Chagas disease patients. PLoS Negl Trop Dis 12:e0006480 (2018). PubMed: 29750791
- Egui A et al. Phenotypic and Functional Profiles of Antigen-Specific CD4+ and CD8+ T Cells Associated With Infection Control in Patients With Cutaneous Leishmaniasis. Front Cell Infect Microbiol 8:393 (2018). PubMed: 30510917
- Mateus J et al. Antiparasitic Treatment Induces an Improved CD8+ T Cell Response in Chronic Chagasic Patients. J Immunol 198:3170-3180 (2017). PubMed: 28258194