PE Anti-pan Cytokeratin抗体[C-11] (ab52460)
![Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)](https://www.abcam.cn/ps/products/52/ab52460/Images/ab52460-265341-anti-pan-cytokeratin-antibody-c-11-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)")
Key features and details
- PE Mouse monoclonal [C-11] to pan Cytokeratin
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
PE Anti-pan Cytokeratin抗体[C-11]
参阅全部 pan Cytokeratin 一抗 -
描述
PE小鼠单克隆抗体[C-11] to pan Cytokeratin -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human pan Cytokeratin. Keratin-enriched preparation from human epidermoid carcinoma cell line A431
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阳性对照
- Flow Cyt (Intra): HT-29 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
C-11 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1 µg/ml.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 1 µg/ml. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 374454 Human
- Entrez Gene: 51350 Human
- SwissProt: Q01546 Human
- SwissProt: Q7Z794 Human
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别名
- pan ck antibody
- pan-ck antibody
- panck antibody
图片
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Flow Cytometry (Intracellular) - PE Anti-pan Cytokeratin antibody [C-11] (ab52460)
Intracellular Flow Cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.
Recommended conc. 0.5 µg/ml
Overlay with Isotype mouse IgG1 control.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab52460 被引用在 18 文献中.
- Vaquero-Siguero N et al. Modeling Colorectal Cancer Progression Reveals Niche-Dependent Clonal Selection. Cancers (Basel) 14:N/A (2022). PubMed: 36077793
- Korangath P & Ivkov R Magnet-assisted Flow Cytometry of in vivo Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles. Bio Protoc 10:e3822 (2020). PubMed: 33659474
- Cheng KS et al. ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells. Theranostics 10:11026-11048 (2020). PubMed: 33042268
- Karin O et al. Senescent cell turnover slows with age providing an explanation for the Gompertz law. Nat Commun 10:5495 (2019). PubMed: 31792199
- Singh M et al. Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration. Cancer Res 79:2962-2977 (2019). PubMed: 30952634