重组PE Anti-NMDAR2B抗体[S59] (ab274365)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Mouse monoclonal [S59] to NMDAR2B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-NMDAR2B抗体[S59]
参阅全部 NMDAR2B 一抗 -
描述
PE小鼠单克隆抗体[S59] to NMDAR2B -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment aa 1-300 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00960 -
阳性对照
- ICC: HaCat cells. WB: HEK-293T NR2B induced lysate; Rat brain lysate IHC-P: Mouse backskin tissue.
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常规说明
The clone number has been updated from S59-36 to N59/36, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
S59 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274365于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
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IHC-P |
Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa). |
IHC-P
Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
组织特异性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
别名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PE Anti-NMDAR2B antibody [S59] (ab274365)
Immunohistochemistry analysis of Bouin’s Fixative-fixed, paraffin-embedded mouse backskin tissue stained for NMDAR2B (Green) using ab93610 at 1/100 dilution, followed by a FITC-conjugated Goat-Anti-Mouse secondary at 1/50 dilution.
This data was developed using the unconjugated version of this antibody (ab93610).
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Immunocytochemistry/Immunofluorescence analysis of cold 100% methanol-fixed for 10 minutes at -20°C, HaCaT cells stained for NMDAR2B (Green) using ab93610 at 1/100 dilution, followed by a FITC-conjugated Goat Anti-Mouse secondary at 1/50 dilution.
This data was developed using the unconjugated version of this antibody (ab93610).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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