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PE Anti-Neutrophil抗体[7/4] (ab105157)

Submit a review Q&A (6)References (5)
Flow Cytometry - PE Anti-Neutrophil antibody [7/4] (ab105157)

    Key features and details

    • PE Rat monoclonal [7/4] to Neutrophil
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    FITC Unconjugated

    概述

    • 产品名称

      PE Anti-Neutrophil抗体[7/4]
      参阅全部 Neutrophil 一抗

    • 描述

      PE大鼠单克隆抗体[7/4] to Neutrophil

    • 宿主

      Rat

    • 偶联物

      PE. Ex: 488nm, Em: 575nm

    • 特异性

      Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Mouse

    • 免疫原

      Tissue, cells or virus corresponding to Mouse Neutrophil. Cultured mouse bone marrow cells.

    • 表位

      Recognizes the Ly-6B.2 antigen.

    • 常规说明

      ab105157 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

      ab105157 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 纯化说明

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

    • 克隆

      单克隆

    • 克隆编号

      7/4

    • 骨髓瘤

      Y3/Ag1.2.3

    • 同种型

      IgG2a

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab105157于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use 10µl for 106 cells.

    ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
    说明
    Flow Cyt
    Use 10µl for 106 cells.

    ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 

    靶标

    • 细胞定位

      Cell membrane; Lipid-anchor, GPI-anchor

    • 数据库链接

      • 别名

        • Ly-6B antibody
        • Ly6b antibody
        • Lymphocyte antigen 6B antibody
        • Lymphocyte antigen 6B Short name=Ly-6B antibody

      图片

      • Flow Cytometry - PE Anti-Neutrophil antibody [7/4] (ab105157)
        Flow Cytometry - PE Anti-Neutrophil antibody [7/4] (ab105157)
        Mouse (strain C57BL6) peripheral granulocytes stained with ab105157.

      文献 (5)

      发表研究结果有使用 ab105157？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab105157 被引用在 5 文献中.

      • Cai Z  et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). PubMed: 27444869
      • Rosas M  et al. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol 88:169-80 (2010). PubMed: 20400676
      • Locke LW  et al. A novel neutrophil-specific PET imaging agent: cFLFLFK-PEG-64Cu. J Nucl Med 50:790-7 (2009). PubMed: 19372473
      • Word RA  et al. Transgene insertion on mouse chromosome 6 impairs function of the uterine cervix and causes failure of parturition. Biol Reprod 73:1046-56 (2005). PubMed: 16034000
      • Hirsch S & Gordon S Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4. Immunogenetics 18:229-39 (1983). PubMed: 6618532

      客户评价及客户问答

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      1-6 of 6 Abreviews or Q&A

      Question

      Our lab has been using your product Rat monoclonal [7/4] to neutrophil (phycoerythrin) (ab105157) for a while. The first two Abs worked well, but the last one we got did not work as good. The flow cytometry could not detect a clear population of neutrophil out of the lysed blood (please see data comparison attached).

      Read More
      Answer

      You contacted Abcam back in November 2011 about a problem with ab105157 in flow cytometry. I was wondering if the problems had been resolved or if you are still having problems with it.

      Please let me know if there is anything I can help you with.

      Read More

      Question

      What is the concentration of this antibody?

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      Answer

      Thank you for your inquiry. The concentration of specific antibody in this product cannot be accurately calculated because of the interference in the reading from the PE protein itself. We can estimate that this product is 0.1mg/ml, but again, this is an estimate. I hope this information helps.  Please contact us with any other questions.    

      Read More

      Question

      Hi I wonder about the quantity of antibody in your ´anti-neutrophil antibody 7/4` product. (ab 105157). Is it comparable to the FITC-conjugated antibody?

      Read More
      Answer

      Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

      Question

      Hello,   Our lab has been using your product Rat monoclonal [7/4] to neutrophil (phycoerythrin) (ab105157) for a while. The first two Abs worked well, but the last one we got did not work as good. The flow cytometry could not detect a clear population of neutrophil out of the lysed blood (please see data comparison attached).   Could it be anything wrong with this specific bottle? 

      Read More
      Answer

      Thank you for contacting us and for including your attached flow data. I agree the vial of ab105157 you received is not working as expected. If you could forward me the purchase order number or Abcam order reference number associated with the purchase I would be happy to send you a replacement.

      Read More

      Question

      Our lab has been using your product Rat monoclonal [7/4] to neutrophil (phycoerythrin) (ab105157) for a while. The first two Abs worked well, but the last one we got did not work as good. The flow cytometry could not detect a clear population of neutrophil out of the lysed blood (please see data comparison attached).   Could it be anything wrong with this specific bottle? 

      Read More
      Answer

      Thank you for contacting Abcam. I am sorry that the current lot of ab105157 is not working in flow cytometry. The data you provide clearly shows that there is a difference in the results between the old and the new antibody. Would you be able to send me the lot number for the current antibody you have and the lot number for the old antibody, if you still have that information? I would like to see if there is any information about these specific lots that could account for such a difference. I look forward to your reply and helping you resolve this issue.

      Read More

      Question

      Dear Sir/ Madam, I am PhD student in Sydney University. I have Rat and Mice paraffin and frozen skin tissue. I want to measure Neutrophils. According to your experience would you please lead me whether ab45977 is suitable primary antibody or not. Thank you for your cooperation. Best Regards,

      Read More
      Answer

      Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying the one as per your requirements. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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