PE Anti-MHC class II I E kappa抗体[14-4-4S] (ab25585)
![Flow Cytometry - PE Anti-MHC class II I E kappa antibody [14-4-4S] (ab25585)](https://www.abcam.cn/ps/products/25/ab25585/Images/ab25585-1-anti-mhc-class-ii-i-e-kappa-antibody-14-4-4s-pe-flow-cytometry-splenocytes-akr-mouse.jpg "Flow Cytometry - PE Anti-MHC class II I E kappa antibody [14-4-4S] (ab25585)")
Key features and details
- PE Mouse monoclonal [14-4-4S] to MHC class II I E kappa
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
PE Anti-MHC class II I E kappa抗体[14-4-4S]
参阅全部 MHC class II I E kappa 一抗 -
描述
PE小鼠单克隆抗体[14-4-4S] to MHC class II I E kappa -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
This antibody is specific to an epitope on mouse I-E kappa MHC class II alloantigen. The antibody reacts with the I-E kappa class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to crossreact with the rat class II alloantigen RT1D.
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经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue/ cell preparation: C3H mouse skin graft and splenocytes.
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阳性对照
- Flow Cyt: AKR mouse splenocytes.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
14-4-4S -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25585于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
靶标
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相关性
A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes. -
细胞定位
Type I membrane protein -
别名
- H2 Ea antibody
- H2-Ea antibody
图片
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Flow Cytometry - PE Anti-MHC class II I E kappa antibody [14-4-4S] (ab25585)
Flow cytometric analysis of AKR mouse splenocytes labeling MHC class II E kappa using ab25585 at 0.1 µg/106 cells in 100 µl. CD3ε is labeled using a Rat Anti-Mouse CD3ε (FITC) antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab25585 被引用在 5 文献中.
- Farr A et al. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol 26:3185-93 (1996). PubMed: 8977321
- Roy M et al. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol 25:596-603 (1995). PubMed: 7533092
- Neiss U & Reske K Non-coordinate synthesis of MHC class II proteins and invariant chains by epidermal Langerhans cells derived from short-term in vitro culture. Int Immunol 6:61-71 (1994). PubMed: 8148327
- Harton JA et al. Structure function analysis of the H-2 Abp gene. Immunogenetics 34:358-65 (1991). PubMed: 1748486
- Ozato K et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). PubMed: 7188699