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AB252136

重组PE Anti-Mesothelin抗体[EPR19025-42]

PE Anti-Mesothelin antibody [EPR19025-42]

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(2 Publications)

Rabbit Recombinant Monoclonal Mesothelin antibody - conjugated to PE. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 2 publications.

查看别名

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

1 Images
Flow Cytometry - PE Anti-Mesothelin antibody [EPR19025-42] (AB252136)
  • Flow Cyt

Unknown

Flow Cytometry - PE Anti-Mesothelin antibody [EPR19025-42] (AB252136)

Overlay histogram showing HeLa cells stained with ab252136 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab252136) (1x 106 in 100μl at 2.5 μg/ml (1/200)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

不同偶联物与剂型 (5)

  • Carrier free

    Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture)

  • Carrier free

    Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free

  • Unconjugated

    Anti-Mesothelin antibody [EPR19025-42]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Mesothelin antibody [EPR19025-42]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Mesothelin antibody [EPR19025-42]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19025-42

亚型

IgG

偶联物

PE

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

No

反应种属

Human, Human

应用

Flow Cyt, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Store in the dark

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
See full target information MSLN

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Immunology 174:310-321 PubMed39746895

2025

Co-Expression of Dominant-Negative TGF-β Receptor 2 Enhances the Therapeutic Efficacy of Novel TREM1/DAP12-BB-Based CAR-T Cells in Solid Tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Sichao Zhu,Jianping Hu,Jie Lin,Chen Wang,Enxiu Wang

Frontiers in oncology 13:1239957 PubMed38162496

2024

BME-free primary patient-specific organoids obtained with a one-day mimicking method to replicate the corresponding tumor for personalized treatment options.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhu,Zhechun Ding,Yini Wang,Qing Wu,Dongmei Chen,Luanhong Wang,Yuancheng Li,Yao Yao,Jiman Huang,Yun Li,Xiaojing Wang,Yanchun Lin,Tian Guan,Haoyu Zeng,Congzhu Li
View all publications

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