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AB318309

重组PE Anti-Ly-6A/E (Sca-1) [EPR22502-38]

PE Anti-Ly-6A/E (Sca-1) [EPR22502-38]

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Rabbit Recombinant Monoclonal Sca1 / Ly6A/E antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples.

查看别名

Ly6, Ly6a, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, T-cell-activating protein, SCA-1, TAP

1 Images
Flow Cytometry - PE Anti-Ly-6A/E (Sca-1) [EPR22502-38] (AB318309)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-Ly-6A/E (Sca-1) [EPR22502-38] (AB318309)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab318309 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab318309 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 106 in 100 μl at 0.04 μg/ml (1/12)) for 30min on ice. The cells were simultaneously stained with c-Kit.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on live cells.

不同偶联物与剂型 (5)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38]

  • 660 APC

    APC Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38]

  • Unconjugated

    Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38]

  • Carrier free

    Anti-Ly-6A/E (Sca-1) antibody [EPR22502-38] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22502-38

亚型

IgG

偶联物

PE

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

No

反应种属

Mouse, Mouse

应用

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/12500", "FlowCyt-species-notes": "<p></p>" } } }

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

T-cell activation.
See full target information Ly6a

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

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