重组PE Anti-Integrin alpha 2抗体[EPR17338] (ab225284)
![Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)](https://www.abcam.cn/ps/products/225/ab225284/Images/ab225284-333782-anti-integrin-alpha-2-antibody-epr17338-phycoerythrin-flow-cytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR17338] to Integrin alpha 2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-Integrin alpha 2抗体[EPR17338]
参阅全部 Integrin alpha 2 一抗 -
描述
PE兔单克隆抗体[EPR17338] to Integrin alpha 2 -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): A431 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17338 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
- Alexa Fluor® 488 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab208770)
- Alexa Fluor® 647 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209766)
- HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
- Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab210655)
- Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
- Anti-Integrin alpha 2 antibody [EPR17338] - BSA and Azide free (ab271936)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225284于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
The cellular localisation of this product has been verified in ICC/IF. |
| 说明 |
|---|
|
Flow Cyt (Intra)
1/1000. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3673 Human
- Entrez Gene: 16398 Mouse
- Entrez Gene: 170921 Rat
- Omim: 192974 Human
- SwissProt: P17301 Human
- SwissProt: Q62469 Mouse
- Unigene: 482077 Human
- Unigene: 5007 Mouse
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别名
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
图片
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Flow Cytometry (Intracellular) - PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
Overlay histogram showing A431 cells stained with ab225284 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225284, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
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![PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)](https://www.abcam.cn/ps/products/225/ab225284/Images/ab225284-3-benefits-of-recombinant-antibodies.png)
PE Anti-Integrin alpha 2 antibody [EPR17338] (ab225284)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab225284 尚未被引用在任何文献中。