重组PE Anti-IL-21R抗体[EPR23637-53]

• Flow Cyt

Lab

Flow Cytometry - PE Anti-IL-21R antibody [EPR23637-53] (AB313962)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab313962 (right) or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab313962 or Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) (1x 10⁶ in 100 μl at 0.2 μg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter. Events were gated on viable lymphocytes.