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PE Anti-HLA Class I抗体[MEM-123] (ab58998)

Submit a review Q&A (1)References (1)
Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)

    Key features and details

    • PE Mouse monoclonal [MEM-123] to HLA Class I
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PE. Ex: 488nm, Em: 575nm
    • Isotype: IgG3

    Conjugates logo Related conjugates and formulations

    Unconjugated

    概述

    • 产品名称

      PE Anti-HLA Class I抗体[MEM-123]
      参阅全部 HLA Class I 一抗

    • 描述

      PE小鼠单克隆抗体[MEM-123] to HLA Class I

    • 宿主

      Mouse

    • 偶联物

      PE. Ex: 488nm, Em: 575nm

    • 特异性

      ab58998 reacts with all human classical MHC Class I molecules in native cell-surface forms as well as with human HLA G cDNA transfected cells. ab58998 completely blocks binding of classical W6/32 to surface expressed HLA G.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human HLA Class I. COS-7 cells
      Database link: P04439

    • 阳性对照

      • Flow Cyt: K562 cells

    • 常规说明

      Do not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

      The purified antibody was conjugated with R Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      pH: 7.4
      Preservative: 0.097% Sodium azide
      Constituent: 0.2% BSA

      0.2% high grade protease free BSA
    • Concentration information loading...

    • 纯度

      Size exclusion

    • 纯化说明

      The antibody was >95% pure as determined from SDS-PAGE (purified using protein A affinity chromatography prior to conjugation).

    • 克隆

      单克隆

    • 克隆编号

      MEM-123

    • 同种型

      IgG3

    • 研究领域

    相关产品

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab58998于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    Use a concentration of 2 - 4 µg/ml.
    说明
    Flow Cyt
    Use a concentration of 2 - 4 µg/ml.

    靶标

    图片

    • Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)
      Flow Cytometry - PE Anti-HLA Class I antibody [MEM-123] (ab58998)
      Flow Cytometry analysis of K562 cells labeling HLA Class I with Anti-HLA Class I antibody [MEM-123] (Phycoerythrin) (ab58998).

    文献 (1)

    发表研究结果有使用 ab58998？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab58998 被引用在 1 文献中.

    • Toyoshima K  et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . PubMed: 25622566

    客户评价及客户问答

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    Question

    Would like to use this antibody in IHC-Fr to stain human cells injected into mouse heart tissue.

    Read More
    Answer


    DISCOUNT CODE: ***
    Expiration date: June 22, 2012

    This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ab58998 in IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com