重组PE Anti-Histone H2A抗体[EPR17470] – ChIP Grade (ab318341)
![PE Anti-Histone H2A antibody [EPR17470] – ChIP Grade (ab318341)](https://www.abcam.cn/ps/products/318/ab318341/Images/ab318341-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "PE Anti-Histone H2A antibody [EPR17470] – ChIP Grade (ab318341)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR17470] to Histone H2A - ChIP Grade
- Suitable for: Antibody labelling, Target binding affinity
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-Histone H2A抗体[EPR17470] – ChIP Grade
参阅全部 Histone H2A 一抗 -
描述
PE兔单克隆抗体[EPR17470] to Histone H2A - ChIP Grade -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Antibody labelling, Target binding affinitymore details -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17470 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318341于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Antibody labelling |
Use at an assay dependent concentration.
|
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| Target binding affinity |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Antibody labelling
Use at an assay dependent concentration. |
|
Target binding affinity
Use at an assay dependent concentration. |
靶标
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功能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
序列相似性
Belongs to the histone H2A family. -
翻译后修饰
The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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别名
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
图片
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PE Anti-Histone H2A antibody [EPR17470] – ChIP Grade (ab318341)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab318341 尚未被引用在任何文献中。