PE Anti-HA tag抗体[16B12] (ab72479)
![Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)](https://www.abcam.cn/ps/products/72/ab72479/Images/ab72479-241227-anti-ha-tag-antibody-16b12-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)")
Key features and details
- PE Mouse monoclonal [16B12] to HA tag
- Suitable for: Flow Cyt
- Reacts with: Species independent
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
概述
-
产品名称
PE Anti-HA tag抗体[16B12]
参阅全部 HA tag 一抗 -
描述
PE小鼠单克隆抗体[16B12] to HA tag -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Species independent -
免疫原
Raised against the twelve amino acid peptide CYPYDVPDYASL.
-
表位
ab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA). -
常规说明
Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.013% Sodium azide
Constituents: 0.1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate -
Concentration information loading... -
纯度
Size exclusion -
纯化说明
Size exclusion chromatography as well as proprietary methods for purifying the final materials were used. -
克隆
单克隆 -
克隆编号
16B12 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
相关性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
别名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
图片
-
Flow Cytometry - PE Anti-HA tag antibody [16B12] (ab72479)
Flow cytometry analysis of a recombinant (N-terminus HA-tag) transmembrane cell surface receptor in CHO cells using ab72479 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab72479 被引用在 2 文献中.
- Takano T et al. Dectin-1 intracellular domain determines species-specific ligand spectrum by modulating receptor sensitivity. J Biol Chem 292:16933-16941 (2017). PubMed: 28848046
- Yugawa T et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). PubMed: 24019071