PE Anti-EGFR抗体[EGFR1] (ab130738)
Key features and details
- PE Mouse monoclonal [EGFR1] to EGFR
- Suitable for: Flow Cyt
- Reacts with: Horse, Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2b
Related conjugates and formulations
概述
-
产品名称
PE Anti-EGFR抗体[EGFR1]
参阅全部 EGFR 一抗 -
描述
PE小鼠单克隆抗体[EGFR1] to EGFR -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab130738 reacts with the extracellular domain of Human EGFR. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Horse, Human
预测可用于: Mouse -
免疫原
Tissue, cells or virus corresponding to Human EGFR. Human epidermoid carcinoma line A431
-
表位
Within amino acids 6-273 -
阳性对照
- Human blood cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
-
纯度
Size exclusion -
纯化说明
Purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. -
克隆
单克隆 -
克隆编号
EGFR1 -
同种型
IgG2b -
研究领域
相关产品
-
Alternative Versions
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130738于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | (1) |
Use 10µl for 106 cells.
or 100 µl of whole blood.
ab91532 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
说明 |
---|
Flow Cyt
Use 10µl for 106 cells. or 100 µl of whole blood.
ab91532 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
靶标
-
功能
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action. -
组织特异性
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. -
疾病相关
Lung cancer
Inflammatory skin and bowel disease, neonatal, 2 -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. -
细胞定位
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). - Information by UniProt
-
数据库链接
- Entrez Gene: 1956 Human
- Entrez Gene: 13649 Mouse
- Omim: 131550 Human
- SwissProt: P00533 Human
- SwissProt: Q01279 Mouse
- Unigene: 488293 Human
- Unigene: 605083 Human
- Unigene: 420648 Mouse
see all -
别名
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
- Cell proliferation inducing protein 61 antibody
see all
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab130738 被引用在 5 文献中.
- Rao TC et al. ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion. J Biol Chem 298:101726 (2022). PubMed: 35157848
- Rebetz J et al. Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One 3:e1936 (2008). PubMed: 18398462
- Fortunel NO et al. Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations. J Cell Sci 116:4043-52 (2003). PubMed: 12953061
- Aigner A et al. Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation. Oncogene 20:2101-11 (2001). PubMed: 11360194
- Akiyama M et al. Changing patterns of localization of putative stem cells in developing human hair follicles. J Invest Dermatol 114:321-7 (2000). PubMed: 10651993