AB209297

重组PE Anti-Cytokeratin 8抗体[EP1628Y]

PE Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 8 antibody - conjugated to PE. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

查看别名

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

2 Images

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

Overlay histogram showing HeLa cells stained with ab209297 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209297, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycorythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

ab209297 staining Cytokeratin 8 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209297 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

不同偶联物与剂型 (6)

Unconjugated

Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y]
HRP

HRP Anti-Cytokeratin 8 antibody [EP1628Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1628Y

亚型

IgG

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

反应种属

Human, Mouse

应用

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/pe-rabbit-igg-monoclonal-epr25a-isotype-control-ab209478'>ab209478</a> - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Do Not Freeze|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Current oncology (Toronto, Ont.) 31:5677-5693 PubMed39330049

2024

A Preliminary Analysis of Circulating Tumor Microemboli from Breast Cancer Patients during Follow-Up Visits.

Applications

Unspecified application

Species

Unspecified reactive species

Hung-Chih Lai,Hsing-Hua Huang,Yun-Jie Hao,Hsin-Ling Lee,Chiao-Chan Wang,Thai-Yen Ling,Jen-Kuei Wu,Fan-Gang Tseng

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组PE Anti-Cytokeratin 8抗体[EP1628Y]