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AB95676

PE/Cy7® Anti-c-Kit抗体[2B8]

PE/Cy7® Anti-c-Kit antibody [2B8]

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(1 Publication)

Rat Monoclonal c-Kit antibody - conjugated to PE/Cy7®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Kit protein.

查看别名

CD117, Sl, Kit, Mast/stem cell growth factor receptor Kit, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit

1 Images
Flow Cytometry - PE/Cy7® Anti-c-Kit antibody [2B8] (AB95676)
  • Flow Cyt

Unknown

Flow Cytometry - PE/Cy7® Anti-c-Kit antibody [2B8] (AB95676)

Flow cytomtry staining of C57BL/6 bone marrow cells with FITC conjugated anti-Human/Mouse CD45R (B220) and (1) PE-Cy7 conjugated Rat IgG2b kappa Isotype Control at 0.5 µg or (2) ab95676 at 0.5 µg. Total viable cells were used for analysis.

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

2B8

亚型

IgG2b

轻链类型

kappa

偶联物

PE/Cy7®

激发波长/发射波长

Ex: 565nm, Em: 778nm

不含载体蛋白

No

反应种属

Mouse

应用

Flow Cyt

applications

免疫原

Cell preparation containing Kit protein. The exact immunogen used to generate this antibody is proprietary information.

P05532

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^5 Cells", "FlowCyt-species-notes": "<p>Cell number should be determined empirically but can range from 10<sup>5</sup> to 10<sup>8</sup> cells/test (based on a test volume of 100 μl).</p><p><a href='/products/primary-antibodies/pe-cy7-rat-igg2b-kappa-monoclonal-klh-g2b-1-2-isotype-control-ab154461'>ab154461</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
pH: 7.2 Preservative: 0.09% Sodium azide
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The c-Kit protein also known as CD117 is a type of receptor tyrosine kinase with a molecular weight of approximately 145 kDa. It is encoded by the KIT gene and commonly expressed on the surface of hematopoietic stem cells mast cells melanocytes and germ cells. C-Kit plays a role in the signal transduction pathways that control cell proliferation and survival. Researchers often identify c-Kit using markers like biotin c-Kit PerCP Cy5.5 or antibodies such as anti-c-Kit for experimental purposes.
Biological function summary

C-Kit influences the function and survival of certain cell types. When it binds to its ligand stem cell factor (SCF) it activates receptor dimerization and phosphorylation triggering downstream signaling. C-Kit does not act alone; it often functions in a complex with other proteins to affect cellular outcomes. Its expression is vital for the maintenance of bone marrow hematopoietic cells and contributes to melanocyte and germ cell development.

Pathways

C-Kit interacts with the PI3K/AKT and RAS/ERK pathways through its kinase activity. These pathways play important roles in cell division differentiation and survival. The c-Abl and ACK2 proteins are closely related to c-Kit function within these signaling networks contributing to regulating cellular responses to external stimuli.

C-Kit mutations are frequently associated with gastrointestinal stromal tumors (GIST) and acute myeloid leukemia (AML). These conditions often involve alterations in c-Kit activity leading to uncontrolled cell growth. While studying GIST researchers have noticed interactions between c-Kit and other proteins like CBL which is involved in the modulation of c-Kit signaling. Understanding these interactions helps in designing targeted therapies for conditions involving c-Kit dysregulation.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
See full target information Kit
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 9:1794 PubMed35071488

2022

Endogenous Aβ induces osteoporosis through an mTOR-dependent inhibition of autophagy in bone marrow mesenchymal stem cells (BMSCs).

Applications

Unspecified application

Species

Unspecified reactive species

Ye Lin,Tianyu Chen,Junjian Chen,Yingying Fang,Canjun Zeng
View all publications
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详情请参阅我们的条款与条件。
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