AB25349

PE/Cy5® Anti-CD45.1抗体[A20]

Name: PE/Cy5® Anti-CD45.1抗体[A20] (ab25349)| Abcam中文官网
Brand: Abcam Limited
SKU: AB25349
Price: 5004 CNY
Availability: InStock

PE/Cy5® Anti-CD45.1 antibody [A20]

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(2 Publications)

Anti-CD45.1 antibody [A20] conjugated to PE/Cy5® - suitable for flow cytometry, and reacts with mouse samples. Clone [A20] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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查看别名

CD45, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA

1 Images

Flow Cytometry - PE/Cy5® Anti-CD45.1 antibody [A20] (AB25349)

Flow Cyt

Supplier Data

Flow Cytometry - PE/Cy5® Anti-CD45.1 antibody [A20] (AB25349)

SJL mouse splenocytes were stained with Mouse Anti- Mouse CDE45.1-PE/Cy5 (ab25349). The grey peak represents an isotype control. The purple peak represents the positive dataset.

不同偶联物与剂型 (1)

Unconjugated

Anti-CD45.1 antibody [A20]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

A20

亚型

IgG2a

轻链类型

kappa

偶联物

PE/Cy5®

激发波长/发射波长

Ex: 496nm, Em: 667nm

不含载体蛋白

反应种属

Mouse

应用

Flow Cyt

applications

特异性

<p>The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD 45.1 allotype.</p>

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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产品详情

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.1 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose, 0.2% Gelatin

运输条件

Blue Ice

储存信息

Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD45.1 also known as Leukocyte Common Antigen (LCA) or Protein Tyrosine Phosphatase Receptor Type C (PTPRC) is a protein expressed on the surface of all nucleated hematopoietic cells. It has a molecular mass of approximately 180-220 kDa. This protein acts as a receptor-type protein tyrosine phosphatase. CD45.1 is an essential marker in the immune system particularly found on T cells B cells and other white blood cells. The expression of CD45.1 is seen across leukocytes where it plays a significant role in cell signaling and antigen receptor signaling.

Biological function summary

CD45.1 influences the immune response by modulating signals through antigen receptors. It operates as a single-pass type I membrane protein and is not known to be part of a larger complex. This protein is important in the activation and regulation of T-cells and B-cells by fine-tuning the threshold of signals required for cell activation. The presence of CD45.1 affects multiple processes in adaptive immunity impacting lymphocyte development and their further activation in immune responses.

Pathways

CD45.1 is integral in lymphocyte activation pathways. It influences the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways by dephosphorylating specific kinases like Lck and Fyn which are important in initiating TCR and BCR signaling cascades. Moreover CD45.1 interacts with other proteins such as CD3 and CD19 being essential in the regulation of immune response and maintaining proper signaling amplitudes to ensure effective immune function.

CD45.1 is intricately linked to autoimmune diseases and leukemia. The protein's dysregulation can lead to improper immune signaling contributing to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In leukemia the altered expression of CD45.1 and related proteins such as CD20 often correlates with disease progression and treatment response. Understanding the roles of CD45.1 can facilitate the development of targeted therapies in these contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.

See full target information Ptprc

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Blood 87:4049-56 PubMed8639760

1996

Enhanced engraftment of hematopoietic progenitor cells in mice treated with granulocyte colony-stimulating factor before low-dose irradiation: implications for gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

M Mardiney,H L Malech

Transplantation 57:249-56 PubMed7906058

1994

The role of host T cell subsets in bone marrow rejection directed to isolated major histocompatibility complex class I versus class II differences of bm1 and bm12 mutant mice.

Applications

Unspecified application

Species

Unspecified reactive species

D A Vallera,P A Taylor,J Sprent,B R Blazar

View all publications

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PE/Cy5® Anti-CD45.1抗体[A20]