AB25531

PE/Cy5® Anti-CD3 epsilon抗体[C363.29B]

Name: PE/Cy5® Anti-CD3 epsilon抗体[C363.29B] (ab25531)| Abcam中文官网
Brand: Abcam Limited
SKU: AB25531
Price: 4983 CNY
Availability: InStock

PE/Cy5® Anti-CD3 epsilon antibody [C363.29B]

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(2 Publications)

Rat Monoclonal CD3E antibody - conjugated to PE/Cy5®. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

查看别名

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

1 Images

Flow Cytometry - PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (AB25531)

Flow Cyt

Unknown

Flow Cytometry - PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (AB25531)

Flow Cytometry analysis of BALB/c mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (PE/Cy5) ab25531 (1 µg/10⁶cells) and Rat Anti-Mouse CD19-PE.

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

C363.29B

亚型

IgG2c

轻链类型

kappa

偶联物

PE/Cy5®

激发波长/发射波长

Ex: 496nm, Em: 667nm

不含载体蛋白

反应种属

Mouse

应用

Flow Cyt

applications

表位

This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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产品详情

In the presence of Fc receptor-bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The antibody is cytolytic, is easily used for cell surface staining. This antibody has shown to be useful in studies of in vitro depletion of CD3+ cells, and in vitro activation of T cells (accessory cells must be provided).

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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性能和储存信息

形式

Liquid

纯度

IgG fraction

存储溶液

pH: 7.1 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose, 0.2% Gelatin

运输条件

Blue Ice

储存信息

Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).

See full target information Cd3e

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70161 PubMed39815665

2025

A Risk Model Based on Ferroptosis-Related Genes OSMR, G0S2, IGFBP6, IGHG2, and FMOD Predicts Prognosis in Glioblastoma Multiforme.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqiu Wu,Ling Liu,Zhili Li,Tian Zhang,Qi Wang,Meixiong Cheng

Brain : a journal of neurology 123 ( Pt 6):1092-101 PubMed10825349

2000

Differential adhesion molecule requirements for immune surveillance and inflammatory recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

M D Carrithers,I Visintin,S J Kang,C A Janeway

View all publications

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Abcam Product Promise

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PE/Cy5® Anti-CD3 epsilon抗体[C363.29B]

PE/Cy5® Anti-CD3 epsilon antibody [C363.29B]

Flow Cytometry - PE/Cy5® Anti-CD3 epsilon antibody [C363.29B] (AB25531)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

表位