PE Anti-CD86抗体[BU63] (ab77226)
![Flow Cytometry - PE Anti-CD86 antibody [BU63] (ab77226)](https://www.abcam.cn/ps/products/77/ab77226/Images/ab77226-273615-anti-cd86-antibody-bu63-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD86 antibody [BU63] (ab77226)")
Key features and details
- PE Mouse monoclonal [BU63] to CD86
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
PE Anti-CD86抗体[BU63]
参阅全部 CD86 一抗 -
描述
PE小鼠单克隆抗体[BU63] to CD86 -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab77226 reacts with CD86 expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD86. B-lymphoblastoid cell line ARH 77
-
常规说明
The antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
纯度
Size exclusion -
纯化说明
The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
克隆
单克隆 -
克隆编号
BU63 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
- APC Anti-CD86 antibody [BU63], prediluted (ab134385)
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- FITC Anti-CD86 antibody [BU63] (ab77276)
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77226于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
| 说明 |
|---|
应用说明
Flow Cyt: The reagent is designed for Flow Cytometry analysis of human blood cells
Use 20 µl/100 µl of whole blood or 106 cells in a suspension.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Use 20 µl/100 µl of whole blood or 106 cells in a suspension.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
组织特异性
Expressed by activated B-lymphocytes and monocytes. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
-
别名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
图片
-
Flow Cytometry - PE Anti-CD86 antibody [BU63] (ab77226)
Analysis by flow cytometry of ab77226 staining CD86 in human peripheral blood cells.
-
![Flow Cytometry - PE Anti-CD86 antibody [BU63] (ab77226)](https://www.abcam.cn/ps/products/77/ab77226/Images/ab77226-92953-anti-cd86-antibody-bu63-phycoerythrin-flow-cytometry.jpg)
Flow Cytometry - PE Anti-CD86 antibody [BU63] (ab77226)Flow cytometry analysis of human peripheral blood leukocytes with anti-human CD86 (BU63) PE.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab77226 被引用在 5 文献中.
- Lei X et al. Mitochondrial Calcium Nanoregulators Reverse the Macrophage Proinflammatory Phenotype Through Restoring Mitochondrial Calcium Homeostasis for the Treatment of Osteoarthritis. Int J Nanomedicine 18:1469-1489 (2023). PubMed: 36998601
- Giguère JF et al. Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol 78:6222-32 (2004). PubMed: 15163715
- Mauri D et al. Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol 155:118-27 (1995). PubMed: 7541409
- Engel P et al. The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood 84:1402-7 (1994). PubMed: 7520767
- Caux C et al. B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med 180:1841-7 (1994). PubMed: 7525840