PE Anti-CD44抗体[MEM-263] (ab58754)
![Western blot - PE Anti-CD44 antibody [MEM-263] (ab58754)](https://www.abcam.cn/ps/products/58/ab58754/Images/ab58754-72668-anti-cd44-antibody-mem-263-phycoerythrin-western-blot.jpg "Western blot - PE Anti-CD44 antibody [MEM-263] (ab58754)")
Key features and details
- PE Mouse monoclonal [MEM-263] to CD44
- Suitable for: WB
- Reacts with: Dog, Human, Pig, African green monkey
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
PE Anti-CD44抗体[MEM-263]
参阅全部 CD44 一抗 -
描述
PE小鼠单克隆抗体[MEM-263] to CD44 -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab58754 reacts with the extracellular (N terminal) domain of standard CD44 present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Dog, Human, Pig, African green monkey -
免疫原
Tissue, cells or virus corresponding to Human CD44. COS-7 cells
Database link: P16070-1 -
阳性对照
- Peripheral blood lymphocytes from human, dog or pig. Kg-1a human acute leukemia cell lysate. JURKAT human leukemia T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
纯度
Size exclusion -
纯化说明
The conjugate is purified by size exclusion chromatography -
克隆
单克隆 -
克隆编号
MEM-263 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58754于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). |
靶标
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功能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
组织特异性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
序列相似性
Contains 1 Link domain. -
结构域
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻译后修饰
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 403939 Dog
- Entrez Gene: 960 Human
- Entrez Gene: 100525150 Pig
- Omim: 107269 Human
- SwissProt: Q28284 Dog
- SwissProt: P16070 Human
- Unigene: 502328 Human
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别名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
图片
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Western blot - PE Anti-CD44 antibody [MEM-263] (ab58754)All lanes : PE Anti-CD44 antibody [MEM-263] (ab58754) at 2 µg/ml
Lane 1 : Human peripheral blood lymphocytes
Lane 2 : Canine peripheral blood lymphocytes
Lane 3 : Porcine peripheral blood lymphocytes
Secondary
All lanes : anti-mouse IgG/HRP
Predicted band size: 82 kDa
Observed band size: 82 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab58754 被引用在 3 文献中.
- Oh J et al. Mesenchymal stem cells genetically engineered to express platelet-derived growth factor and heme oxygenase-1 ameliorate osteoarthritis in a canine model. J Orthop Surg Res 16:43 (2021). PubMed: 33430899
- Karakas N et al. Neurons from human mesenchymal stem cells display both spontaneous and stimuli responsive activity. PLoS One 15:e0228510 (2020). PubMed: 32407317
- Linard C et al. Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs. Stem Cells Transl Med 2:916-27 (2013). Pig . PubMed: 24068742