PE Anti-CD32B + CD32A抗体[AT10] (ab30357)
![Flow Cytometry - PE Anti-CD32B + CD32A antibody [AT10] (ab30357)](https://www.abcam.cn/ps/products/30/ab30357/Images/ab30357-40339-anti-cd32-antibody-at10-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD32B + CD32A antibody [AT10] (ab30357)")
Key features and details
- PE Mouse monoclonal [AT10] to CD32B + CD32A
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
PE Anti-CD32B + CD32A抗体[AT10] -
描述
PE小鼠单克隆抗体[AT10] to CD32B + CD32A -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
According to the CD32 Cluster workshop (Leucocyte Typing V, pages 823 and 828), clone AT10 reacts equally well with both Fc gamma RII a and b-expressing cells. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD32A. K562 cell line
-
阳性对照
- Human peripheral blood granulocytes
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
AT10 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30357于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use 10µl for 106 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
细胞定位
CD32B: Cell membrane. CD32A: Cell membrane. -
数据库链接
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Omim: 146790 Human
- Omim: 604590 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
- Unigene: 352642 Human
- Unigene: 654395 Human
see all
图片
-
Flow Cytometry - PE Anti-CD32B + CD32A antibody [AT10] (ab30357)
Flow cytometric analysis of human peripheral blood granulocytes with 10µl for 106 cells of anti human CD32A + CD32B Phycoerythrin conjugated (ab30357).
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab30357 被引用在 9 文献中.
- Verhulst S et al. Gene Signatures Detect Damaged Liver Sinusoidal Endothelial Cells in Chronic Liver Diseases. Front Med (Lausanne) 8:750044 (2021). PubMed: 34746184
- Lambrecht J et al. A PDGFRß-based score predicts significant liver fibrosis in patients with chronic alcohol abuse, NAFLD and viral liver disease. EBioMedicine 43:501-512 (2019). PubMed: 31036530
- Coll M et al. Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis. Cell Stem Cell 23:101-113.e7 (2018). PubMed: 30049452
- Sahin A et al. Development of third generation anti-EGFRvIII chimeric T cells and EGFRvIII-expressing artificial antigen presenting cells for adoptive cell therapy for glioma. PLoS One 13:e0199414 (2018). PubMed: 29975720
- Tzeng SJ et al. Fc?RIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK. J Biomed Sci 22:87 (2015). PubMed: 26475492
- Guimarães EL et al. P311 modulates hepatic stellate cells migration. Liver Int N/A:N/A (2014). PubMed: 25243526
- Metes D et al. Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood 91:2369-80 (1998). PubMed: 9516136
- Lilliehook I et al. Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies. Vet Immunol Immunopathol 61:181-93 (1998). PubMed: 9613433
- Greenman J et al. Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol Immunol 28:1243-54 (1991). PubMed: 1835758