AB25380

PE Anti-CD3 epsilon抗体[C363.29B]

Name: PE Anti-CD3 epsilon抗体[C363.29B] (ab25380)| Abcam中文官网
Brand: Abcam Limited
SKU: AB25380
Price: 2303 CNY
Availability: InStock

PE Anti-CD3 epsilon antibody [C363.29B]

Be the first to review this product! Submit a review

(1 Publication)

Rat Monoclonal CD3E antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

查看别名

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

1 Images

Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (AB25380)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (AB25380)

BALB/c lymph node cells were double stained with ab25380 and with anti-IL2RB (ab24943) at 1μg/10⁶ cells. Small lymphocytes were gated and analyzed on a flow cytometer.

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

C363.29B

亚型

IgG2c

轻链类型

kappa

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

反应种属

Mouse

应用

Flow Cyt

applications

表位

The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

style

left-column

产品详情

In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. The monoclonal antibody is cytolytic, easily used for cell surface staining.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose

运输条件

Blue Ice

储存信息

Store in the dark

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).

See full target information Cd3e

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of leukocyte biology 94:1243-51 PubMed23935208

2013

G protein-coupled receptor kinase-3-deficient mice exhibit WHIM syndrome features and attenuated inflammatory responses.

Applications

Unspecified application

Species

Mouse

Teresa K Tarrant,Matthew J Billard,Roman G Timoshchenko,Marcus W McGinnis,D Stephen Serafin,Oded Foreman,Denise A Esserman,Nelson J Chao,William E Lento,David M Lee,Dhavalkumar Patel,David P Siderovski

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD3 epsilon抗体[C363.29B]

PE Anti-CD3 epsilon antibody [C363.29B]

Flow Cytometry - PE Anti-CD3 epsilon antibody [C363.29B] (AB25380)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

表位