PE Anti-CD22抗体[RFB4] (ab23620)
Key features and details
- PE Mouse monoclonal [RFB4] to CD22
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
概述
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产品名称
PE Anti-CD22抗体[RFB4]
参阅全部 CD22 一抗 -
描述
PE小鼠单克隆抗体[RFB4] to CD22 -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
CD22 is in principle a B lineage antigen, that is present in the cytoplasm of progenitor B-cells and on the membrane of the majority of mature peripheral B-lymphocytes. Furthermore, it is strongly expressed on Hairy Cell Leukemia cells and very weakly on some other leukemias. Antigen distribution: Peripheral blood lymphocytes 12±3% T-cells (E+) < 1% B-cells (E-, Ig+) 92±3% Monocytes (CD14+) < 1% Granulocytes < 1% Thymocytes < 1% -
经测试应用
适用于: IHC-Fr, Flow Cytmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Purified tonsil lymphocytes (Human).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯化说明
Purification by IEP (immunoelectrophoresis). -
Primary antibody说明
ab23620 can be used for: - Quantitative determination of peripheral B-cells (CD22+) in blood. - Identification of CD22+-cells in tissue sections. - Determination of B-cell origin of lymphoid neoplasms. - Quantitative elimination or isolation of B-cells by flow cytometry or magnet beads. -
克隆
单克隆 -
克隆编号
RFB4 -
同种型
IgG1 -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23620于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
Use maximum of 5ul for 10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. Use maximum of 5ul for 10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
应用说明
Is unsuitable for IHC-P.
靶标
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功能
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. -
组织特异性
B-lymphocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻译后修饰
Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 933 Human
- Omim: 107266 Human
- SwissProt: P20273 Human
- Unigene: 579691 Human
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别名
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab23620 被引用在 2 文献中.
- Ito D et al. A double blinded, placebo-controlled pilot study to examine reduction of CD34 +/CD117 +/CD133 + lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma. F1000Res 4:42 (2015). PubMed: 28357033
- Ito D et al. Exclusion of cytoplasmic fragments in flow cytometric analysis of lymph node samples from dogs with lymphoma using membrane-permeable violet laser-excitable DNA-binding fluorescent dye (DyeCycle Violet). Vet Clin Pathol 39:494-8 (2010). PubMed: 21198735