PE Anti-CD22抗体[MEM-01]，prediluted (ab27548)
![Flow Cytometry - PE Anti-CD22 antibody [MEM-01], prediluted (ab27548)](https://www.abcam.cn/ps/products/27/ab27548/Images/ab27548-38347-anti-cd22-antibody-mem-01-prediluted-phycoerythrin-flow-cytometry.jpg "Flow Cytometry - PE Anti-CD22 antibody [MEM-01], prediluted (ab27548)")
Key features and details
- PE Mouse monoclonal [MEM-01] to CD22, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
概述
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产品名称
PE Anti-CD22抗体[MEM-01]，prediluted
参阅全部 CD22 一抗 -
描述
PE小鼠单克隆抗体[MEM-01] to CD22，prediluted -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Non human primates
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免疫原
Tissue, cells or virus corresponding to CD22. Burkitt's lymphoma cell line Raji
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
High grade protease free BSA -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
MEM-01 -
同种型
IgG1 -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27548于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 20µl for 106 cells.
Use 20µl for 106 cells or 100µl of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
Flow Cyt
Use 20µl for 106 cells. Use 20µl for 106 cells or 100µl of whole blood. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. -
组织特异性
B-lymphocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
结构域
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻译后修饰
Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 933 Human
- Omim: 107266 Human
- SwissProt: P20273 Human
- Unigene: 579691 Human
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别名
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
图片
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Flow Cytometry - PE Anti-CD22 antibody [MEM-01], prediluted (ab27548)ab27548 staining CD22 on human peripheral blood cells. Cell population was gated on lymphocytes.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab27548 尚未被引用在任何文献中。