AB27548

PE Anti-CD22抗体[MEM-01]，prediluted

Name: PE Anti-CD22抗体[MEM-01] (ab27548)| Abcam中文官网
Brand: Abcam Limited
SKU: AB27548
Price: 1666 CNY
Availability: InStock

PE Anti-CD22 antibody [MEM-01]

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(1 Publication)

Mouse Monoclonal CD22 antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD22 protein.

查看别名

CD22, SIGLEC2, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, BL-CAM, Siglec-2

1 Images

Flow Cytometry - PE Anti-CD22 antibody [MEM-01] (AB27548)

Flow Cyt

Unknown

Flow Cytometry - PE Anti-CD22 antibody [MEM-01] (AB27548)

ab27548 staining CD22 on human peripheral blood cells. Cell population was gated on lymphocytes.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-01

亚型

IgG1

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Cell preparation containing CD22 protein. The exact immunogen used to generate this antibody is proprietary information.

P20273

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "Use 20μl for 106 cells or 100μl of whole blood.<a href='/products/primary-antibodies/pe-mouse-igg1-b11-6-isotype-control-ab91357'>ab91357</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯度

IgG fraction

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD22 also known as Siglec-2 or B-lymphocyte cell adhesion molecule (BL-CAM) is a transmembrane protein with a mass of around 140 kDa. It is selectively expressed on the surface of mature B cells and some immature B cells. This protein plays an important role in the immune system particularly in the regulation of B cell function. CD22 functions by modulating signal transduction processes through its cytoplasmic domain which contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs).

Biological function summary

CD22 acts mainly as a negative regulator of B cell receptor (BCR) signaling. It is part of a complex that interacts with the BCR and other accessory molecules. CD22 helps maintain B cell tolerance by inhibiting overactive signaling and preventing autoimmune responses. By binding to sialic acid-containing glycans CD22 modulates interactions between B cells and other cells aiding in the maintenance of immune homeostasis.

Pathways

CD22 plays a critical role in the BCR signaling pathway and the regulation of phosphatidylinositol 3-kinase (PI3K) signaling. It works together with proteins like CD19 and SHP-1 that are key components of these pathways. By influencing these signaling cascades CD22 contributes to the modulation of B cell activation proliferation and apoptosis ensuring proper functioning of the immune response.

CD22 is associated with autoimmune conditions and hematological malignancies such as hairy cell leukemia (HCL). Therapeutic approaches targeting CD22 such as anti-CD22 antibodies are being explored for treating these diseases. CD22's role in these conditions is connected with proteins involved in the autoimmune pathways or cancer development offering potential therapeutic targets for intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed : 34330755, PubMed : 8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed : 20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed : 8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed : 20516366).

See full target information CD22

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 198:1453-62 PubMed14610046

2003

LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nadezda Brdicková,Tomás Brdicka,Pavla Angelisová,Ondrej Horváth,Jiri Spicka,Ivan Hilgert,Jan Paces,Luca Simeoni,Stefanie Kliche,Camilla Merten,Burkhart Schraven,Václav Horejsí

View all publications

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Abcam Product Promise

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PE Anti-CD22抗体[MEM-01]，prediluted

PE Anti-CD22 antibody [MEM-01]

Flow Cytometry - PE Anti-CD22 antibody [MEM-01] (AB27548)

关键信息

宿主种属

克隆

克隆号

亚型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原