AB324033

PE Anti-CD21+CD35 antibody [EPR27417-513]

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal CD21 antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples.

CD21, Complement receptor type 2, Cr2, Complement C3d receptor

3 Images

Flow Cytometry - PE Anti-CD21+CD35 antibody [EPR27417-513] (AB324033)

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD21+CD35 antibody [EPR27417-513] (AB324033)

Flow cytometric analysis of mouse splenocytes labelling CD35 + CD21 with ab324033 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) / Left isotype control.

Cells are co-stained with CD19 conjugated to Alexa Fluor®647.
Gated on viable cell.

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD21+CD35 antibody [EPR27417-513] (AB324033)

Cells are co-stained with CD3 conjugated to Alexa Fluor®488.
Gated on viable cell.

Flow Cyt

Supplier Data

Flow Cytometry - PE Anti-CD21+CD35 antibody [EPR27417-513] (AB324033)

Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / WEHI-231 (mouse B cell lymphoma B lymphocyte, Right) cells labelling CD35 + CD21 with ab324033 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cell.
Negative control : EL4(PMID : 10453011)

不同偶联物与剂型 (3)

Unconjugated

Anti-CD21+CD35 antibody [EPR27417-513]
Carrier free

Anti-CD21+CD35 antibody [EPR27417-513] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR27417-513

亚型

IgG

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

反应种属

Mouse, Mouse

应用

ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Also induces specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.

See full target information Cr2

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PE Anti-CD21+CD35 antibody [EPR27417-513]