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AB213186

重组PE Anti-CD11b抗体[EP1345Y]

PE Anti-CD11b antibody [EP1345Y]

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(4 Publications)

Rabbit Recombinant Monoclonal CD11b antibody - conjugated to PE. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

查看别名

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

1 Images
Flow Cytometry - PE Anti-CD11b antibody [EP1345Y] (AB213186)
  • Flow Cyt

Lab

Flow Cytometry - PE Anti-CD11b antibody [EP1345Y] (AB213186)

Overlay histogram showing human CD14+ monocytes stained with ab213186 (red line). Human peripheral blood mononuclear cells were incubated for 30 min on ice in 1x PBS containing 10ug/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions. Then cells were surface stained for CD14 FITC (ab28061) for 30 min on ice. The cells were then permeabilised with 4.2% formaldehyde and 0.1% saponin for 20 min at 4°C. After cells were blocked with 1xPBS containing 0.1% saponin and 10% normal goat serum for 30 min on ice followed by the antibody (ab213186, 1x106 cells in 100ul at 1ug/ml) for 30 min on ice.

Isotype control antibody (black line) Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. CD14 FITC single stained cells (blue line) were also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. Events were gated on live single cell CD14+ positive monocytes.

不同偶联物与剂型 (3)

  • Unconjugated

    Anti-CD11b antibody [EP1345Y] - C-terminal

  • Carrier free

    Anti-CD11b antibody [EP1345Y] - BSA and Azide free

  • 660 APC

    APC Anti-CD11b antibody [EP1345Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1345Y

亚型

IgG

偶联物

PE

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

No

反应种属

Human, Human

应用

Flow Cyt, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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替代产品

Primary Antibodies

AB184308

Anti-CD11b antibody [EPR19387]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (AB184308)

  • 4
  • 1
Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
Primary Antibodies

AB216445

Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (AB216445)

  • 0
  • 0

搭配使用产品

Assay Kits

AB277412

Human CD11b ELISA Kit

ELISA - Human CD11b ELISA Kit (AB277412)

  • 0
  • 0
Primary Antibodies

AB280849

Alexa Fluor® 555 Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD4 antibody [EPR6855] (AB280849)

  • 5
  • 2
Primary Antibodies

AB303467

Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB225472

Alexa Fluor® 488 Anti-Granzyme B antibody [EPR20129-217]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Granzyme B antibody [EPR20129-217] (AB225472)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
Up to 12 months
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen and factor X. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrin ITGAM/ITGB2 is also a receptor for ICAM1 ligand ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
See full target information ITGAM
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:493-500 PubMed40836086

2025

Structural basis for the dynamic regulation of mTORC1 by amino acids.

Applications

Unspecified application

Species

Unspecified reactive species

Max L Valenstein,Maximilian Wranik,Pranav V Lalgudi,Karen Y Linde-Garelli,Yuri Choi,Raghu R Chivukula,David M Sabatini,Kacper B Rogala

Journal of orthopaedic surgery and research 18:937 PubMed38062424

2023

Exosomal miR-182 derived from bone marrow mesenchymal stem cells drives carfilzomib resistance of multiple myeloma cells by targeting SOX6.

Applications

Unspecified application

Species

Unspecified reactive species

Shifeng Long,Shengping Long,Honglei He,Liang Luo,Mei Liu,Ting Ding

Bioengineered 12:12891-12904 PubMed34784837

2021

MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyan Sun,Zhihe Jiang,Yanling Chen,Di Shang,Pan Miao,Jian Gao

Journal of nanobiotechnology 19:194 PubMed34193158

2021

Mesenchymal stem cell-derived extracellular vesicles prevent the development of osteoarthritis via the circHIPK3/miR-124-3p/MYH9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglong Li,Jie Liu,Siyu Liu,Weijie Jiao,Xiaohong Wang
View all publications
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