AB212007

重组PE Anti-Caveolin-1抗体[EPR15554] - N-terminal

PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal Caveolin-1 antibody - conjugated to PE. N-terminal. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

查看别名

CAV, CAV1, Caveolin-1

1 Images

Flow Cytometry (Intracellular) - PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB212007)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker (AB212007)

Overlay histogram showing A549 cells stained with ab212007 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab212007, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

不同偶联物与剂型 (4)

Carrier free

Anti-Caveolin-1 antibody [EPR15554] - BSA and Azide free
Unconjugated

Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Caveolin-1 antibody [EPR15554] - N-terminal - Caveolae Marker

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15554

亚型

IgG

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

反应种属

Human, Human, Mouse

应用

IHC-P, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Do Not Freeze|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-1 also known as Cav-1 is an integral membrane protein with a molecular weight of approximately 22 kDa. It functions mechanically as a scaffolding protein in caveolae which are tiny invaginations in the plasma membrane of many cell types. These structures are particularly abundant in adipocytes endothelial cells and muscle cells. Caveolin-1 anchors itself to the cell membrane and plays a role in organizing and concentrating certain signaling molecules.

Biological function summary

Caveolae provide a platform for various signaling pathways and involve caveolin-1 as a major component. Caveolin-1 interacts with multiple signaling molecules such as G-protein coupled receptors and Src family kinases to modulate signal transduction. This protein forms part of a larger caveolar complex contributing to cellular processes including endocytosis and lipid regulation. Its presence as a marker in caveolae highlights its significance in cellular functions.

Pathways

Caveolin-1 influences the insulin signaling and nitric oxide (NO) signaling pathways. In the insulin signaling pathway caveolin-1 interacts with insulin receptors to modulate glucose uptake. It also associates with eNOS (endothelial nitric oxide synthase) in the NO signaling pathway impacting vascular function. These relationships highlight its role in cellular communication and regulatory mechanisms within the human body.

Caveolin-1 is involved in cancer and cardiovascular diseases. In cancer altered expression of caveolin-1 contributes to tumor progression while in cardiovascular diseases it affects endothelial function through its interaction with eNOS. The connection of caveolin-1 to proteins like insulin receptors and eNOS reveals its critical influence in these conditions providing potential targets for therapeutic intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May act as a scaffolding protein within caveolar membranes (PubMed : 11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed : 19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed : 25893292). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).

See full target information CAV1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1583271 PubMed40421296

2025

Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Qianren Zheng,Jie Chen,Yixun Huang,Weikai Chen,Dandan Cheng,Qianqian Jia,Meiqin Zhu,Youguo Liao,Qiulin He,Shunli Wu

eLife 9: PubMed32234209

2020

Functionally heterogeneous human satellite cells identified by single cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Barruet,Steven M Garcia,Katharine Striedinger,Jake Wu,Solomon Lee,Lauren Byrnes,Alvin Wong,Sun Xuefeng,Stanley Tamaki,Andrew S Brack,Jason H Pomerantz

View all publications

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