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AB305731

重组PE Anti-c-Myc (phospho S62)抗体[EPR17924]

PE Anti-c-Myc (phospho S62) antibody [EPR17924]

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Rabbit Recombinant Monoclonal C-Myc/MYC phospho S62 antibody - conjugated to PE. Suitable for Flow Cyt (Intra) and reacts with Human samples.

查看别名

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

1 Images
Flow Cytometry (Intracellular) - PE Anti-c-Myc (phospho S62) antibody [EPR17924] (AB305731)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-c-Myc (phospho S62) antibody [EPR17924] (AB305731)

Flow cytometry overlay histogram showing left untreated HeLa cells and right HeLa cells treated with 4000U/mL Lambda Protein Phosphatase for 2 hours at 30°C stained with ab305731 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab305731) (1x 106 in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin Isotype Control (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.

This antibody gave a positive signal in cells fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

不同偶联物与剂型 (10)

  • 660 APC

    APC Anti-c-Myc (phospho S62) antibody [EPR17924]

  • HRP

    HRP Anti-c-Myc (phospho S62) antibody [EPR17924]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-c-Myc (phospho S62) antibody [EPR17924]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-c-Myc (phospho S62) antibody [EPR17924]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-c-Myc (phospho S62) antibody [EPR17924]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-c-Myc (phospho S62) antibody [EPR17924]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-c-Myc (phospho S62) antibody [EPR17924]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-c-Myc (phospho S62) antibody [EPR17924]

  • Unconjugated

    Anti-c-Myc (phospho S62) antibody [EPR17924]

  • Carrier free

    Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17924

亚型

IgG

偶联物

PE

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

No

反应种属

Human

应用

Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).
See full target information MYC phospho S62
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搭配使用产品

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Primary Antibodies

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  • 0
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Secondary Antibodies

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  • 5
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Primary Antibodies

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BOND RX™ Validated|Lab Essentials

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