AB208747

重组PE Anti-Bcl-XL抗体[E18]

Name: PE Anti-Bcl-XL抗体[E18] (ab208747)| Abcam中文官网
Brand: Abcam Limited
SKU: AB208747
Price: 5077 CNY
Availability: InStock
Rating: 4 (2 reviews)

PE Anti-Bcl-XL antibody [E18]

RabMAb
Recombinant
了解详情

(2 Reviews)

(2 Publications)

Anti-Bcl-XL antibody [E18] - PE conjugated (ab208747) is a rabbit recombinant monoclonal antibody detecting Bcl-XL in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

查看别名

BCL2L, BCLX, BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X

3 Images

Flow Cytometry (Intracellular) - PE Anti-Bcl-XL antibody [E18] (AB208747)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - PE Anti-Bcl-XL antibody [E18] (AB208747)

Overlay histogram showing HeLa cells stained with ab208747 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208747, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Flow Cytometry - PE Anti-Bcl-XL antibody [E18] (AB208747)

Flow Cyt

Lab

Flow Cytometry - PE Anti-Bcl-XL antibody [E18] (AB208747)

Flow cytometric analysis of 4% paraformaldehyde fixed NIH/3T3 (mouse embryonic fibroblast) cells (red line), permeabilized with 90% methanol. Isotype control : rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black). Cell without incubation with primary antibody (Blue).

Flow Cyt

Lab

Flow Cytometry - PE Anti-Bcl-XL antibody [E18] (AB208747)

Flow cytometric analysis of 4% paraformaldehyde fixed C6 (rat glial tumor glial cell) (red line), permeabilized with 90% methanol. Isotype control : rabbit IgG monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Black). Cell without incubation with primary antibody (Blue).

不同偶联物与剂型 (7)

Unconjugated

Anti-Bcl-XL antibody [E18]
Carrier free

Anti-Bcl-XL antibody [E18] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Bcl-XL antibody [E18]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Bcl-XL antibody [E18]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Bcl-XL antibody [E18]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Bcl-XL antibody [E18]
HRP

HRP Anti-Bcl-XL antibody [E18]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

E18

亚型

IgG

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

不含载体蛋白

反应种属

Mouse, Rat, Human, Human, Mouse, Rat

应用

Flow Cyt (Intra), ICC/IF, IHC-P, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody should recognize Bcl-XL, Bcl-xS and Bcl-x(beta) as the immunogen sequence is common to them. The antibody does not cross-react with other Bcl-2 family members.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
PE Anti-Bcl-XL antibody [E18] (ab208747) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Related products
Antibody clone E18 is also available pre-conjugated to a variety of labels for your convenience – Anti-Bcl-XL PE [E18] (ab208747).

Other related products
We have a range of other formats of antibody clone [E18] also available for your convenience: ab32370, Carrier free - ab199099, HRP - ab200600, PE - ab208747, Alexa Fluor® 647 - ab303457, Alexa Fluor® 555 - ab306571, Alexa Fluor® 750 - ab321595

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Do Not Freeze|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.

Biological function summary

This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.

Pathways

The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.

Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).. Isoform Bcl-X(S) promotes apoptosis.

See full target information BCL2L1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Viruses 14: PubMed35746739

2022

Intraneuronal β-Amyloid Accumulation: Aging HIV-1 Human and HIV-1 Transgenic Rat Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Hailong Li,Kristen A McLaurin,Charles F Mactutus,Benjamin Likins,Wenfei Huang,Sulie L Chang,Rosemarie M Booze

Molecular therapy : the journal of the American Society of Gene Therapy 29:47-59 PubMed33010232

2020

Augmentation of NK Cell Proliferation and Anti-tumor Immunity by Transgenic Expression of Receptors for EPO or TPO.

Applications

Unspecified application

Species

Unspecified reactive species

Chantiya Chanswangphuwana,David S J Allan,Mala Chakraborty,Robert N Reger,Richard W Childs

View all publications

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重组PE Anti-Bcl-XL抗体[E18]