重组PE Anti-Aldolase抗体[EPR23181-39] (ab275163)
![Flow Cytometry (Intracellular) - PE Anti-Aldolase antibody [EPR23181-39] (ab275163)](https://www.abcam.cn/ps/products/275/ab275163/Images/ab275163-1-ab275163RabbitmonoclonalEPR2318139toALDOARPEHumanA549cellsflowcytometry.jpg "Flow Cytometry (Intracellular) - PE Anti-Aldolase antibody [EPR23181-39] (ab275163)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR23181-39] to Aldolase
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-Aldolase抗体[EPR23181-39]
参阅全部 Aldolase 一抗 -
描述
PE兔单克隆抗体[EPR23181-39] to Aldolase -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt: A549
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23181-39 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Aldolase antibody [EPR23181-39] (ab252953)
- Anti-Aldolase antibody [EPR23181-39] - BSA and Azide free (ab273636)
- Alexa Fluor® 488 Anti-Aldolase antibody [EPR23181-39] (ab275161)
- Alexa Fluor® 647 Anti-Aldolase antibody [EPR23181-39] (ab275162)
- APC Anti-Aldolase antibody [EPR23181-39] (ab319313)
- Alexa Fluor® 594 Anti-Aldolase antibody [EPR23181-39] (ab319806)
- Alexa Fluor® 555 Anti-Aldolase antibody [EPR23181-39] (ab319947)
- Alexa Fluor® 750 Anti-Aldolase antibody [EPR23181-39] (ab321498)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275163于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/12500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/12500. |
靶标
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功能
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. -
通路
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
疾病相关
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis. -
序列相似性
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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数据库链接
- Entrez Gene: 226 Human
- Omim: 103850 Human
- SwissProt: P04075 Human
- Unigene: 513490 Human
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别名
- ALDA antibody
- Aldo1 antibody
- ALDOA antibody
see all
图片
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Flow Cytometry (Intracellular) - PE Anti-Aldolase antibody [EPR23181-39] (ab275163)
Flow cytometry overlay histogram showing A549 cells stained with ab275163 (red line). The cells were fixed with 80% methanol (5 min); and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab275163) (1x 106 in 100μl at 0.04μg/ml (1/12500)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 585/42 bandpass filter.
This antibody gave a positive signal in A549 cells fixed with 4% formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab275163 尚未被引用在任何文献中。