Anti-PDZRN3抗体(ab272628)
Key features and details
- Rabbit polyclonal to PDZRN3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PDZRN3抗体 -
描述
兔多克隆抗体to PDZRN3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PDZRN3 aa 750-900.
Database link: Q9UPQ7 -
阳性对照
- WB: RT4, U-251MG sp. IHC-P: Human duodenum tissue. ICC/IF: U-2 OS.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
E3 ubiquitin-protein ligase. Plays an important role in regulating the surface level of MUSK on myotubes. Mediates the ubiquitination of MUSK, promoting its endocytosis and lysosomal degradation. Might contribute to terminal myogenic differentiation. -
组织特异性
Widely expressed, including in the heart, skeletal muscle and liver and, at lower levels, in the brain, colon, small intestine, placenta and lung. Down-regulated in ovarian serous papillary tumors. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 2 PDZ (DHR) domains.
Contains 1 RING-type zinc finger.
Contains 1 TRAF-type zinc finger. -
结构域
The RING-type zinc finger domain is required for E3 ligase activity. -
翻译后修饰
Auto-ubiquitinated. - Information by UniProt
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数据库链接
- Entrez Gene: 23024 Human
- Omim: 609729 Human
- SwissProt: Q9UPQ7 Human
- Unigene: 660347 Human
- Unigene: 662049 Human
- Unigene: 666839 Human
- Unigene: 743998 Human
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别名
- E3 ubiquitin-protein ligase PDZRN3 antibody
- KIAA1095 antibody
- Ligand of Numb protein X 3 antibody
see all
图片
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Western blot - Anti-PDZRN3 antibody (ab272628)Lanes 2-3 : Anti-PDZRN3 antibody (ab272628) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Lane 3 : U-251MG sp -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZRN3 antibody (ab272628)Paraffin-embedded formalin-fixed Human duodenum tissue stained for PDZRN3 using ab272628 at 1/200 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PDZRN3 antibody (ab272628)PFA fixed, Triton X-100 permeablized human cell line U-2 OS labeling PDZRN3 (green) using ab272628 at 2 ug/ml in ICC/IF analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272628 尚未被引用在任何文献中。