Anti-PDXK.1抗体(ab251802)
Key features and details
- Rabbit polyclonal to PDXK.1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-PDXK.1抗体
参阅全部 PDXK.1 一抗 -
描述
兔多克隆抗体to PDXK.1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human PDXK.1 aa 100-250.
Database link: O00764 -
阳性对照
- IHC-P: Human testis, kidney, cerebral cortex and skeletal muscle tissues. ICC/IF: U-2 OS cells. WB: RT4, U-251 MG, NIH/3T3 and NBT-II cell lysate; Human plasma, liver and tonsil lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab251802于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
|
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| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for synthesis of pyridoxal-5-phosphate from vitamin B6. -
组织特异性
Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa. -
序列相似性
Belongs to the pyridoxine kinase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8566 Human
- Entrez Gene: 216134 Mouse
- Entrez Gene: 83578 Rat
- Omim: 179020 Human
- SwissProt: O00764 Human
- SwissProt: Q8K183 Mouse
- SwissProt: O35331 Rat
- Unigene: 284491 Human
see all -
别名
- pdxK antibody
- PDXK_HUMAN antibody
- PKH antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-PDXK.1 antibody (ab251802)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PDXK.1 (green) using ab251802 at 4 µg/ml in ICC/IF.
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Western blot - Anti-PDXK.1 antibody (ab251802)All lanes : Anti-PDXK.1 antibody (ab251802) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (Rat Wistar bladder tumour cells) cell lysate
Predicted band size: 35 kDa -
Western blot - Anti-PDXK.1 antibody (ab251802)All lanes : Anti-PDXK.1 antibody (ab251802) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 35 kDaWestern Blot analysis of ab251802 (A) shows a similar pattern to an independent antibody (B).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab251802)Paraffin-embedded human skeletal muscle tissue stained for PDXK.1 using ab251802 at 1/1000 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab251802)Paraffin-embedded human cerebral cortex tissue stained for PDXK.1 using ab251802 at 1/1000 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab251802)Paraffin-embedded human kidney tissue stained for PDXK.1 using ab251802 at 1/1000 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK.1 antibody (ab251802)Paraffin-embedded human testis tissue stained for PDXK.1 using ab251802 at 1/1000 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab251802 尚未被引用在任何文献中。